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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EPA OPPTS 835.3120 (Sealed Vessel Carbon Dioxide Production Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran
EC Number:
262-475-2
EC Name:
Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran
Cas Number:
60857-05-8
Molecular formula:
C10H16O
IUPAC Name:
4-methylidene-2-(2-methylprop-1-en-1-yl)oxane
Details on test material:
- Chemical name of test material (tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran
- Lot/batch No.: LJ 1-28/29
- Analytical purity: 99.2 area-%

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge taken from municipal a waste water treatment plant (Mannheim, Germany)
- Concentration of sludge: 4 mg/l
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 mg/L
Based on:
other: TOC
Initial conc.:
25 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
TOC removal
Details on study design:
TEST CONDITIONS
- Test temperature: 22 ± 2 °C
- pH-value: 7.4 +/- 0.2

TEST SYSTEM
- Test vessels: 160 mL incubation bottles filled up to a volume of 100 mL
- Bottles were closed with butyl rubber septa gas tight and shaken by an orbital shaker with a speed of about 150 r/min
- Sampling: at sampling day three bottles of each test assays were sacrificed
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (TOC removal)
Value:
10 - 20
Sampling time:
28 d

Applicant's summary and conclusion

Interpretation of results:
other: not readily biodegradable

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