Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: RELIABLE SUMMARY REPORT.
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
review article or handbook
Title:
HEAVY FUEL OILS. ROBUST SUMMARY OF INFORMATION ON HEAVY FUEL OILS.
Author:
AMERICAN PETROLEUM INSTITUTE
Year:
2003
Bibliographic source:
HEAVY FUEL OILS. ROBUST SUMMARY OF INFORMATION ON HEAVY FUEL OILS. PG 21

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
96 h

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LL0
Effect conc.:
>= 1 000 mg/L
Conc. based on:
not specified
Basis for effect:
mortality

Any other information on results incl. tables

No fish exposed to WAF of light fuel oil died during the test. 96-hr LL0 = 1000 mg/l based on nominal loading rates. After 96 h, 1 of the 7 control fish died. All fish in the 100 mg/l treatment exhibited no toxic symptoms. All fish in the 1000 mg/l WAF showed abnormal swimming. Total peak area of the dissolved components of each batch of freshly prepared WAFs was similar. Peak area values ranged from 19-21 x 108 at loading rate of 1000 mg/l and 9-11 x 108 at 100 mg/l. Peak profile was different at different loading rates but peak profile for new and old media was similar. Mean reduction in total peak area was 27% during the test (range 5 - 47%). Peak profiles for the WAFs differed significantly from profile of light fuel oil in dichloromethane. Only two loading rates were tested which is less than a minimum of five concentrations stated in the guidelines. Water hardness was higher than targeted range of 50 - 250 mg/l as CaCO3. Hardness range of 286 - 292 mg/l as CaCO3 was normal for this laboratory and did not adversely affect the health of the fish.

Applicant's summary and conclusion