Tricyclodecanedimethanol

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given; reliable source

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Objective of study:

metabolism

Principles of method if other than guideline:

Identification and quantification of metabolites in an in vitro biodegradation test (MITI (I) test) of the test substance.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Tricyclodecanedimethanol

EC Number:

248-096-5

EC Name:

Tricyclodecanedimethanol

Cas Number:

26896-48-0

Molecular formula:

C12H20O2

IUPAC Name:

[3-(hydroxymethyl)tricyclo[5.2.1.0²,⁶]decan-8-yl]methanol

Details on test material:

- Name of test material (as cited in study report): (Tricyclo[5.2.1.0(2,6)]decane-4,8-diyl)dimethanol; 4,8-Dihydroxymethyl-tricyclo[5.2.1.0(2,6)]decane, Dimethyloltricyclodecane
- Analytical purity: no data
- Composition of test material, percentage of components: test substance was a mixture showing 7 peaks in HPLC
- Stability under test conditions: no data
- Storage condition of test material: no data

Test animals

Species:

other: bacterial culture (mixture of activated sludge from various sources - sewage plants, rivers, lake, bays)

Strain:

other: not specified various bacterial strains

Administration / exposure

Route of administration:

other: incubation of test substance with activated sludge in culture medium for 4 weeks

Vehicle:

unchanged (no vehicle)

Duration and frequency of treatment / exposure:

4 weeks, single dose

Doses / concentrations

Remarks:

Doses / Concentrations:
100 mg TS/L reaction medium

No. of animals per sex per dose / concentration:

not applicatble

Details on dosing and sampling:

METABOLITE CHARACTERISATION
- culture medium was analyzed by LC-MS

Results and discussion

Metabolite characterisation studies

Metabolites identified:

yes

Details on metabolites:

partial oxidation of the test substance resulting in the formation of 2-7% of an aldehyde (oxidation of one of the hydroxymethyl groups, molecular weight = 194) and 12-15% of a carboxylic acid (further oxidation of the formyl group, molecular weight = 210)

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): other: Test substance is metabolized by bacteria in a similar way as by mammalian cells
Octahydro-4,7-methano-1H-indenedimethanol is primarily metabolized by oxidation of one hydroxy group of the methylol substiuent to aldehyde and further oxidation to the corresponding carboxylic acid.

Executive summary:

In a bacterial suspension (MITI (I) biodgradation test), octahydro-4,7-methano-1H-indenedimethanol was primarily metabolized by oxidation of the hydroxy group of one methylol substiuent to aldehyde (2 -7%) and further oxidation to the corresponding carboxylic (NITE, 2004). Thus bacterial metabolism follows the same metabolic pathway for alcohols as is typically found in mammalian systems.