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EC number: 911-490-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4th - 27th February, 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- (2004)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- (2008)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO International Standard 6341: "Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus - Acute toxicity test (1996)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations sampled for analysis
Range-finding test: 10 mg/L
Final test: 0 (blank-control), 32, 56 and 100 mg/L
- Sampling
Frequency: at t=0 h and t=48 h
Volume: 2 ml
- Sample storage conditions before analysis: Samples were stored in a freezer until analysis (≤ -15°C). On the day of analysis, the samples were defrosted at room temperature. If necessary, the samples were diluted with ISO-medium to obtain concentrations within the calibration range. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The preparation of test solutions started with the highest test concentration. No special treatment other than a short magnetic stirring period was applied to fully dissolve the test substance in test medium. The lower test concentrations were prepared by subsequent dilutions in test medium.
- Controls: blank control (test medium without test substance or other additives)
- Evidence of undissolved material: The final test solutions were all clear and colourless. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: In-house laboratory culture with a known history (at least third generation, obtained by acyclical parthenogenesis under specified breeding conditions)
- Age at study initiation: <24 h
- Method of breeding: Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood. Each batch was started with newborn daphnids, i.e. less than 3 days old. M7 medium (according to: Elendt, B.-P., 1990 Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma 154, 25-33) was used. Maximum age of the cultures was 4 weeks.
- Type of food and feeding frequency during breeding: suspension of fresh water algae, feed daily. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 180 mg/L expressed as CaCO3
- Test temperature:
- - Final test:
At start temperature varied between 19.9 and 20.3°C
The temperature continuously measured during the test varied between 18.9 and 19.6°C - pH:
- - Final test
At start: 7.9
At the end: 8.1 - Dissolved oxygen:
- - Final test (mg/L)
At start: 9.9
At the end: 9.2 - 9.3 - Nominal and measured concentrations:
- - Nominal
Range-finding test: 0 (blank-control), 0.1, 1, 10 and 100 mg/L
Final test: 0 (blank-control), 10, 18, 32, 56 and 100 mg/L
- Measured: See the "Remarks on results including figures and tables" field for details. - Details on test conditions:
- TEST SYSTEM
- Test vessel: All-glass, with a capacity of 100 ml and filled with 80 ml of test solution
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates)
Range-finding test: 4 replicates for the 100 mg/L and 2 replicates for 0.1, 1.0 and 10 mg/L
Final test: 4 replicates
- No. of vessels per control (replicates):
Range-finding test: 4 replicates
Final test: 4 replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water purified by Reverse Osmosis
- Breeding medium: M7
- Test medium: Adjusted ISO medium
- Intervals of water quality measurement
Range-finding test: Dissolved oxygen concentrations and pH were only measured in the control and the highest test concentration
Final test:
pH and dissolved oxygen - At the beginning and at the end of the test, for all concentrations and the control.
Temperature of medium - Continuously in a temperature control vessel, beginning at the start of the test.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility (including mortality) at 24 and 48 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. X1.8
- Range finding study
- Test concentrations: 0 (blank-control), 0.1, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 48 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- (including mortality)
- Remarks on result:
- other: 95% CL: 27 to 75 mg/L
- Details on results:
- - Range-finding test: No immobility was observed at concentrations of 0.10, 1.0 and 10 mg/L. The immobility at the highest concentration was 90% after 48 hours. Therefore, the expected EC50 was between 10 and 100 mg/L.
- Final test: The responses recorded were in agreement with what was expected based on the results of the range-finding test. Accelerator (PT 25E or PT 25E/2) did not induce significant acute immobilisation of Daphnia magna at 32 mg/L after 48 hours of exposure (NOEC)
- Acceptability of the test:
1. In the control, no daphnids became immobilised.
2. The oxygen concentration at the end of the test was ≥ 3 mg/L in control and test vessels. - Results with reference substance (positive control):
- Potassium dichromate (K2Cr2O7): The 48h-EC50 was 0.53 mg/L with a 95% confidence interval between 0.49 and 0.62 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study Accelerator (PT 25E or PT 25E/2) did not induce significant acute immobilisation of Daphnia magna at 32 mg/L after 48 hours of exposure (NOEC). The 48h-EC50 was 48 mg/L based on analytically confirmed nominal exposure concentrations (95% confidence interval between 27 and 75 mg/L).
- Executive summary:
In a 48-h acute toxicity study conducted according to OECD guideline 202, water fleas (Daphia magna) were exposed to Accelerator (PT 25E or PT 25E/2) under static conditions at the following nominal concentrations: 0 (blank-control), 10, 18, 32, 56 and 100 mg/L. Accelerator (PT 25E or PT 25E/2) did not induce significant acute immobilisation of Daphnia magna at 32 mg/L after 48 hours of exposure. The 48h-EC50 was 48 mg/L based on analytically confirmed nominal exposure concentrations (95% confidence interval between 27 and 75 mg/L). The study is considered to be reliable without restrictions.
Reference
- Range-finding test
Measured concentrations:
Concentrations of the test substance in test medium in the range-finding test
Time of sampling |
Date of sampling |
Date of analysis1 |
Concentration |
Relative to nominal |
Relative to initial |
||
Nominal |
Analysed |
Analysed corrected2 |
|||||
|
|
|
|
|
|
|
|
0 |
04 -Feb-2013 |
11 -Feb-2013 |
10 |
11.4 |
10.0 |
100 |
|
|
|
|
|
|
|
|
|
48 |
06 -Feb-2013 |
11 -Feb-2013 |
10 |
11.4 |
10.1 |
101 |
100 |
|
|
|
|
|
|
|
|
1 Samples were stored in the freezer (≤ -15°C) until the day of analysis.
2 Corrected for the 113% mean recovery of all procedural recovery samples analysed in this series.
Biological results:
Incidence of immobility in the range-finding test
Concentration Accelerator (PT 25E or PT 25E/2) (mg/L) |
Vessel number
|
Number Daphnia exposed |
Response at 24 h |
Response at 48 h |
||
number |
Total % |
number |
Total % |
|||
Control |
A B C D |
5 5 5 5 |
0 0 0 0 |
0 |
0 0 0 0 |
0 |
0.1 |
A B |
5 5 |
0 0 |
0 |
0 0 |
0 |
1.0 |
A B |
5 5 |
0 0 |
0 |
0 0 |
0 |
10 |
A B |
5 5 |
0 0 |
0 |
0 0 |
0 |
100 |
A B C D |
5 5 5 5 |
3 2 1 1 |
35 |
5 4 5 4 |
90 |
- Final test
Measured concentrations:
Concentrations of the test substance in test medium - final test
Time of sampling |
Date of sampling |
Date of analysis1 |
Concentration |
Relative to nominal |
Relative to initial |
||
Nominal |
Analysed |
Analysed corrected2 |
|||||
|
|
|
|
|
|
|
|
0 |
25 -Feb-2013 |
04 -Mar-2013 |
0 |
n.d. |
n.d. |
n.a. |
|
|
|
|
32 |
37.4 |
33.6 |
105 |
|
|
|
|
56 |
64.8 |
58.3 |
104 |
|
|
|
|
100 |
116 |
104 |
104 |
|
|
|
|
|
|
|
|
|
48 |
27 -Feb-2013 |
04 -Mar-2013 |
0 |
n.d. |
n.d. |
n.a. |
|
|
|
|
32 |
37.4 |
33.6 |
105 |
100 |
|
|
|
56 |
64.7 |
58.2 |
104 |
100 |
|
|
|
100 |
116 |
104 |
104 |
100 |
|
|
|
|
|
|
|
|
1 Samples were stored in the freezer (≤ -15°C) until the day of analysis.
2 Corrected for the 111% mean recovery of the 100 mg/l procedural recovery samples analysed in this series.
n.d. Not detected.
n.a. Not applicable.
Biological results:
Acute immobilisation of daphnids after 24 and 48 hours in the final test
Concentration Accelerator (PT 25E or PT 25E/2) (mg/L) |
Vessel number
|
Number Daphnia exposed |
Response at 24 h |
Response at 48 h |
||
number |
Total % |
number |
Total % |
|||
Control |
A B C D |
5 5 5 5 |
0 0 0 0 |
0 |
0 0 (2) 0 0 |
0 |
10 |
A B C D |
5 5 5 5 |
0 0 0 0 |
0 |
0 0 0 0 |
0 |
18 |
A B C D |
5 5 5 5 |
0 0 0 0 |
0 |
0 0 0 0 |
0 |
32 |
A B C D |
5 5 5 5 |
0 0 1 0 (1) |
5 |
0 0 1 0 |
5 |
56 |
A B C D |
5 5 5 5 |
1 1 1 4 |
35 |
4 5 4 5 |
90 |
100 |
A B C D |
5 5 5 5 |
1 1 1 3 |
30 |
5 3 5 4 |
85 |
( ) between brackets: number of daphnia observed trapped at the surface of the test solutions. These organisms were reimmersed into the respective solutions before recording of mobility.
Description of key information
In a 48-h acute toxicity study conducted according to OECD guideline 202, water fleas (Daphia magna) were
exposed to Accelerator (PT 25E or PT 25E/2). The 48h-EC50 was 48 mg/L based on analytically confirmed
nominal exposure concentrations. The study is considered to be reliable without restrictions.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 48 mg/L
Additional information
In a 48-h acute toxicity study conducted according to OECD guideline 202, water fleas (Daphia magna) were exposed to Accelerator (PT 25E or PT 25E/2) under static conditions at the following nominal concentrations: 0 (blank-control), 10, 18, 32, 56 and 100 mg/L. Accelerator (PT 25E or PT 25E/2) did not induce significant acute immobilisation of Daphnia magna at 32 mg/L after 48 hours of exposure. The 48h-EC50 was 48 mg/L based on analytically confirmed nominal exposure concentrations (95% confidence interval between 27 and 75 mg/L). The study is considered to be reliable without restrictions.
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