Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28.01.2014 - 30.01.2014
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Test organisms (species):
Daphnia magna
Test type:
static
Limit test:
no
Total exposure duration:
48 h
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
20 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: calculated
Basis for effect:
mobility
Remarks on result:
other: not determinable
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
20 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: calculated
Basis for effect:
mobility
Remarks on result:
other: not determinable
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
37 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Calculated
Basis for effect:
mobility
Remarks on result:
other: not determinable
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
28 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: calculated
Basis for effect:
mobility
Remarks on result:
other: not determinable
Validity criteria fulfilled:
yes
Remarks:
No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met. The result of the test is considered valid.
Conclusions:
The following results were determined for the test item Tar wood (species:Daphnia magna).24h-NOEC = 20 mg/L48h-NOEC = 20 mg/L24h-EC50i= 37 mg/L48h-EC50i= 28 mg/L
Executive summary:

Findings and Results:

 

One experiment was performed.

The study was performed using five concentrations ranging from 10 to 100 mg/L nominal load. Twenty Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

After 48 hours, the concentrations showed toxicity between 0% and 95% immobilisation. None of the animals were immobilised in the control.

Potassium dichromate K2Cr2O7(CAS No. 7778-50-9) was used as positive control in a current reference study (201308R201). The value was determined as 1.4 mg/L, lying within the demanded range of 0.6 – 1.7 mg/L. 

At the beginning and at the end of the test, the content of the test item in the test solutions was estimated by calculation from the content of organic carbon of the test item (59.85 %) and DOC measurement. The calculated test item concentrations were in the range of 38 % to 76 % of the nominal concentration. Therefore, the determination of the biological results was based on the geometric mean of the measured concentrations.

Description of key information

The following results were determined for the test item Tar wood (species: Daphnia magna). 

24h-NOEC = 20 mg/L
48h-NOEC = 20 mg/L
24h-EC50i = 37 mg/L
48h-EC50i = 28 mg/L

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
37 mg/L

Additional information

The study was performed using five concentrations ranging from 10 to 100 mg/L nominal load. Twenty Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

The concentrations showed toxicity between 0 and 95% immobilisation. None of the animals were immobilised in the control.

For the estimation of the EC50s the fits showed sufficient statistical correspondence of the data with the dose-response-equation.

At the beginning and at the end of the test, the content of the test item in the test solutions was estimated using DOC-determination. The measured concentrations were in the range of 38 % - 76 % of the nominal concentration. Therefore, the determination of the biological results was based on the geometric mean of the measured concentrations.

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study (201308R201). The value was determined as 1.4 mg/L, lying within the demanded range of 0.6 – 1.7 mg/L.

No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met. 

The result of the test is considered valid.