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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The results of the study showed that under valid static test conditions, the maximum concentration causing no mortality and the 96 h-LC50 of the test item to zebra fish are both greater than the nominal concentration of 150mg/L (measured concentration 147 mg/L).

Key value for chemical safety assessment

Additional information

The acute toxicity of test item (meta-Diisopropanolbenzene) to zebra fish (Brachydanio rerio) under static conditions, was assessed according to “The guidelines for the testing of chemicals”, SEPA(HJ/T 153-2004); and with reference to Procedure 203 of the “Guidelines for Testing of Chemicals” of the OECD: "Fish, Acute Toxicity Test” (1992).

 

A limit test was performed with 100 mg/L and 150 mg/L. Water samples taken from the control and the test treatments in the limit test were analysed. The mean analyzed concentrations were 93.9 mg/L and 147 mg/L respectively. The analytical results showed that the concentration of the test item is consistent in the test medium throughout the 96-hour test period (deviation within 20%).

 

During the whole test period, all fish in the control groups and treated groups were alive and appeared normal. The results showed that under valid static test conditions, the maximum concentration causing no mortality and the 96 h-LC50of the test item to zebra fish are both greater than the nominal concentration of 150mg/L (measured concentration 147 mg/L).

 

The study met the acceptability criteria prescribed by the protocol (pH: 6.0~8.5; dissolved oxygen concentration: > 60% of the air saturation value; total hardness:10~250 mg(CaCO3)/L; temperature: 23±2°C.