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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Version / remarks:
Directive 79/831/EEC, Annex V, CY, Part IV, Manometric Respirometry
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide
EC Number:
406-640-0
EC Name:
A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide
Cas Number:
136920-07-5
Molecular formula:
C58H114N4O6 - C154H308N6O4
IUPAC Name:
Reaction mass of 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C12-C18)alkylacetamide and {[2-(Carboxymethyl-di(C12-C18)alkylcarbamoylmethyl-amino)-ethyl]-di(C12-C18)alkylcarbamoylmethyl-amino}-acetic acid
Constituent 2
Reference substance name:
Keroflux ES 3241
IUPAC Name:
Keroflux ES 3241
Details on test material:
Batch No.: Batch 1
Project No.: 89/0316 rank 1

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
The activated sludge inoculum used in this study originated from laboratory wastewater treatment plants fed with municipal sewage. The concentration of dry substance was 30 mg/L.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100.8 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
0 - 10
Sampling time:
28 d
Details on results:
Abiotic processes in a batch without inoculum:
O2*100/COD = 2 %

BOD5 / COD results

Results with reference substance:
Kinetic of control substance (in %):
= 63 after 9 day(s)

Any other information on results incl. tables

- The inoculum was not significantly inhibited by the test substance
- Abiotic processes in a batch without inoculum: 2 % 

Pretests:
---------
Elementary analysis C = 79.5 % C

BOD = 0 mg/g
COD = 2221 mg/g
ThOD = 2024 mg/g

Applicant's summary and conclusion

Interpretation of results:
under test conditions no biodegradation observed