3- Hydroxy-4-methyoxypyrdine-2-carboxylic acid

Toxicity to terrestrial arthropods

Administrative data

Endpoint:

toxicity to bees: acute oral

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 213 (Honeybees, Acute Oral Toxicity Test)

Deviations:

no

GLP compliance:

yes

Application method:

oral

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxy-4-methoxypyridine-2-carboxylic acid

Cas Number:

210300-09-7

Molecular formula:

C7H7NO4

IUPAC Name:

3-hydroxy-4-methoxypyridine-2-carboxylic acid

Specific details on test material used for the study:

Substance name: X696476
Lot #: XS9-109113-80 (TSN300901)
Purity: 100%

Sampling and analysis

Analytical monitoring:

not specified

Test substrate

Vehicle:

yes

Remarks:

50 % w/v sucrose solution (500 g sucrose/L tap water)

Test organisms

Test organisms (species):

Apis mellifera

Animal group:

Hymenoptera (honeybees)

Study design

Study type:

laboratory study

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

25°C

Humidity:

40-63%

Photoperiod and lighting:

24 h darkness

Nominal and measured concentrations:

14.2 μg a.i./bee.
The dose levels are adjusted to reflect the percentage a.i. (13 μg a.i. = 13 μg (analytical)). The limit dose level is the result of the maximum achievable solubility of the test item in sugar solution.

Reference substance (positive control):

yes

Remarks:

dimethoate

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LD50

Effect conc.:

> 14.2 other: μg a.i./bee

Nominal / measured:

meas. (not specified)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Results with reference substance (positive control):

The oral LD50 (24 h) value of the reference item (dimethoate) was calculated to be 0.15 μg a.i./bee (assessed in a different study).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

48 h LD50 (honey bee): >14.2 µg a.i./bee (oral)

Executive summary:

A study was performed to assess the acute oral toxicity of the test substance to honey bees (Apis mellifera) in accordance with the guideline OECD 213.

In the oral toxicity test the maximum nominal test level of the test substance (13 μg a.i./bee) corresponded to an actual intake of 14.2 μg a.i./bee. This dose level led to 4.0 % mortality after 48 hours. 6.0 mortality occurred in the water control group (50 % w/v sucrose solution = 500 g sucrose/L tap water).

No test item induced behavioural effects were observed at any time in the oral test. The oral LD50 (24 h) value of the reference item (dimethoate) was calculated to be 0.15 μg a.i./bee.

The toxicity of the test substance was tested in an acute oral toxicity test on honey bees. The LD50 (48 h) was > 14.2 μg a.i./bee in the oral toxicity test.