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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

water solubility
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)

Data source

Reference Type:
study report

Materials and methods

Test guidelineopen allclose all
according to guideline
EU Method A.6 (Water Solubility)
according to guideline
OECD Guideline 105 (Water Solubility)
GLP compliance:
Type of method:
flask method

Test material

Constituent 1
Chemical structure
Reference substance name:
ethyl (2S)-3-[3,5-diamino-4-(4-methoxyphenoxy)phenyl]-2-acetamidopropanoate
Cas Number:
Molecular formula:
ethyl (2S)-3-[3,5-diamino-4-(4-methoxyphenoxy)phenyl]-2-acetamidopropanoate
Test material form:
solid: particulate/powder

Results and discussion

Water solubility
Key result
Water solubility:
0.288 g/L
Conc. based on:
test mat.
Loading of aqueous phase:
100 mg/L
20 °C
>= 5 - <= 6
Details on results:
Seven vessels (blank, flasks 1C (for the sampling point 72 h) and flasks 2 – 6) were set onto the shaking apparatus immediately and shaking was started. After 24 ± 2 hours, flask 1B (for the sampling point 48 h) and after 48 ± 2 hours, flask 1A (for the sampling point 24 h) were set on the shaker and all flasks were shaken for further 24 ± 2 hours at room temperature.
Then, the blank and flasks 1A-C were taken from the shaker and tempered to 20.0 ± 0.5 °C. Samples were taken, membrane filtrated with PTFE filters 0.45 µm pore size and pre-pared for HPLC analysis (see chapter 4.6.3).
Because of a difference of less than 15 % in the concentrations on days 2 and 3 and no upward tendency, the test was finished on day 3. The final measurement of flasks 2 – 6 was performed on day 4 for organizational reasons.
All filtrated test solutions showed no Tyndall effect (i.e. a laser beam was not scattered when transmitted through the liquid).

Applicant's summary and conclusion

The solubility of L-Tyrosine, N-acetyl-3,5-diamino-O-(4-methoxyphenyl)-, ethyl ester in wa-ter was determined from the measured concentrations of test item in the filtered test item solutions.
Equilibration time can be considered as approx. 1 day, as the values which were measured on the following consecutive days did not give significantly different values.
All replicates showed good correspondence. The mean test item concentration of the sam-ples (flask 2 - 6), which were used for the calculation of water solubility (at plateau) was 0.288 ± 0.006 g/L (mean ± standard deviation), corresponding to a relative standard devia-tion of 2.2 %.

No observations arousing doubts concerning the accuracy of the results and the validity of the study were made.