Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-379-6 | CAS number: 614-33-5
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Remarks:
- This is an in-vitro test which adheres to generally accepted in-vitro validity criteria
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guideline
- Qualifier:
- no guideline required
- Principles of method if other than guideline:
- The detailed method followed, is described in XCellR8 SOP L0064 "Neutral Red Uptake (NRU) Cytoxicity Test Using Human Dermal Fibroblasts in Xeno-Free Culture Conditions"
- GLP compliance:
- no
- Test type:
- other: In-vitro: Neutral Red Uptake (NRU) Cytoxicity Test Using Human Dermal Fibroblasts in Xeno-Free Culture Conditions
Test material
- Reference substance name:
- Glycerol tribenzoate
- EC Number:
- 210-379-6
- EC Name:
- Glycerol tribenzoate
- Cas Number:
- 614-33-5
- Molecular formula:
- C24H20O6
- IUPAC Name:
- 1,3-bis(benzoyloxy)propan-2-yl benzoate
- Test material form:
- solid: flakes
- Details on test material:
- room temperature: off-white coloured flakes or granulate; > 80 °C: colourless to yellowish liquid
Batch no. 20118
CAS no. 614-33-5
Production date 28. Jul. 2020
Expiry date 15. Aug. 2022
Constituent 1
- Specific details on test material used for the study:
- Batch: 20118
Purity: 98.1%
Expiry: 24 months from 07/2020
Test animals
- Species:
- other: Human Dermal Fibroblast Cells
- Strain:
- other: Neonatal human dermal fibroblast cultures
- Sex:
- not specified
Administration / exposure
- Route of administration:
- other: A single application of 8 concentratons of the test item (n=6) was applied in cell culture medium with 1% DMSO (dilution factor of 2 in the range-finder, 1.3 in the main test).
Results and discussion
- Preliminary study:
- Cell viability did not reduce to below 50% at 200 ug/mL.
Effect levels
- Key result
- Sex:
- not specified
- Dose descriptor:
- other: IC50
- Effect level:
- > 500 other: ug/mL
- Based on:
- test mat.
- Remarks on result:
- other: Based on cell viability
Applicant's summary and conclusion
- Conclusions:
- Glycerol tribenzoate was classified as Category 4 or Category 5 or Unclassified (Classification, IC50>500 ug/mL)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
