Amines, N-C10-16-alkyltrimethylenedi-, reaction products with chloroacetic acid

Endpoint summary

Additional information

Experimental terrestrial data are available for the different trophic levels testing the toxicity of Amines, N-C10-C16-alkyltrimethylenedi-, reaction products with chloroacetic acid on soil invertebrates, plants and microorganisms.

The most sensitive endpoint was determined in soil microorganisms in experiments performed according to OECD 216 and 217. Nitrogen and carbon transformation by soil microorganisms was investigated according to the OECD guidelines 216 and 217. The test item was incorporated into loamy sand soil at nominal concentrations of 1.64, 4.10, 10.24, 25.6, 64, 160, 400, and 1000 mg a.i./kg dry mass at test start. A recalculation revealed that inhibitory effects can be seen at 160 mg a.i./kg dry soil after 28 days. Therefore, the NOEC is 64 mg a.i./kg dry soil.

In a long-term study with invertebrate species Eisenia fetida effects on mortality and biomass were determined after 28 days, the effects on reproduction were determined after 56 days. The test item was applied in concentrations of 313, 625, 1250, 2500 and 5000 mg/per kg dry mass soil, representing 60.9, 122, 244, 488 and 975 mg a.i./kg, respectively. The nominal NOEC for reproduction was found to be the most sensitive assessed endpoint with 1250 mg/kg dry soil, representing 244 mg a.i./kg. No effects were detected in a short-term study with E. fetida according to OECD Guideline 207 and EC method C.8. up to a concentration of 1000 mg a.i./kg.

 

Two short-term and one long-term study are available testing the toxicity of Amines, N-C10-C16-alkyltrimethylenedi-, reaction products with chloroacetic acid to terrestrial plants.

The key short-term study assessed the effects on the emergence and growth of terrestrial plant seedlings according to OECD guideline 208. Concentration related inhibition of plant growth was observed in Avena sativa (NOEC = 400 mg a.i./kg), Phaseolus aureus (NOEC ≤ 1.64 mg a.i./kg), Raphanus sativus (NOEC = 10.2 mg a.i./kg), Lactuca sativa (NOEC = 160 mg a.i./kg), and Sinapis alba (NOEC = 400 mg a.i./kg). However, for the first three of these five species, the effects were only moderate, with the growth rate never being inhibited by more than 50 %. Accordingly, the EC50 values for these tested species were found to be > 1000 mg a.i./kg dry soil. For Lactuca sativa and Sinapis alba, EC50 values of 363 mg a.i./kg and 400–1000 mg a.i./kg, respectively, were found.

 

Another short-term toxicity test tested the effects on Amines, N-C10-C16-alkyltrimethylenedi-, reaction products with chloroacetic acid according to the OECD guideline 208. The relevant NOEC for the most sensitive endpoint (growth of Raphanus sativus) was found to be 1515 mg test item per kg dry soil, representing 295 mg a.i./kg. The EC50was calculated to be 4109 mg test item per kg dry soil, representing 801 mg a.i./kg. According to these results, the test item had a mild inhibitory effect on growth in one of the two plant species tested. With respect to seedling emergence and post-emergence survival, no inhibitory effect occurred.

 

The plant long term-study assessed the effects on the emergence, growth and fertility of terrestrial plant seedlings. The most sensitive NOEC for shoot growth (both, shoot length and fresh weight) of B. rapa and A. sativa at test end were 2500 and 1250 mg test item per kg dry soil, representing 488 and 244 mg a.i./kg, respectively.