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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20 Aug - 23 Aug 2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Qualifier:
according to
Guideline:
other: OECD Guidance Document No. 23 on Aquatic Toxicity of Difficult Substances and Mixtures, OECD 2000
Qualifier:
according to
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
according to
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
GLP compliance:
yes (incl. certificate)
Remarks:
Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance is a mixture that is poorly soluble in water. Therefore the test solution(s) were prepared following general guidance provided in OECD 23 in order to achieve a water accommodated fraction (WAF) of the test substance. The test solution was prepared by directly adding test substance to test medium. A defined volume of test media was placed into a beaker (2L nominal volume) and a glass sampling tube is suspended in the beaker with the top of the tube sufficiently above the surface of the test medium to avoid overflow from the surrounding surface layer. The test substance was pipetted carefully (with a positive displacement pipette), on the water surface outside of the glass tube. The beakers were covered with Parafilm and shaken gently (approx. 50 RPM, to avoid emulsion formation) for about one day. After shaking, the required volume of aqueous fraction was drawn off for testing via the glass sampling tube. The exposure was started after separation of the undissolved material.
Undissolved test substance was visible outside the glass sampling tube at the water surface in the treatment group. The test solution removed from the sampling tube was visibly colorless and clear with no visible undissolved substance.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Source: originally obtained from the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of the BASF SE, Ludwigshafen, Germany
- Age at study initiation: <24 h old (at least 3rd brood progeny)
- Method of breeding: Daphnia brood stock are kept in mass cultures consisting of approx. 20 – 30 parthenogenetically reproducing females for a
maximum of 4 weeks and fed live unicellular algae (Desmodesmus subspicatus), cultured continuously at the test facility. All individuals in each mass culture originate from a single female and are thus genetic clones. After approximately 14 days the adults have produced at least 3 broods and the
young can be used in tests. Offspring are removed from the mass cultures at least once daily during the normal work week to ensure that young daphnia are <24 h old (first instar) at test initiation.
- Feeding during test: none

ACCLIMATION
- Acclimation conditions (same as test or not): The Daphnia are cultured under identical conditions as those in the final test, including test media (Elendt M4), water quality, and temperature (20 ± 1 °C)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
2.51 mmol/L (medium)
Test temperature:
19.5 - 20.6 °C
pH:
7.9 - 8.0
Dissolved oxygen:
8.1 - 8.6 mg O2/L
Nominal and measured concentrations:
Nominal: control, 100 mg/L (WAF, limit test)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): Closed
- Material, size, headspace, fill volume: Test tubes (glass) with flat bottom (nominal volume 20 mL) filled with 10 mL test solution
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water (Elendt M4) is used as media for culture and test purposes according to OECD guideline 202.
- Total organic carbon: 1 mg/L
- Ca/Mg ratio: about 4 : 1
- Conductivity: 636µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: 147 – 666 lux at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilisation of Daphnia is determined after 24 and 48 hours of exposure.

TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: In a preliminary range-finding test (conducted under non-GLP conditions) a concentration of 100 mg/L caused no immobilisation or any other symptoms after 48 hours. According to the OECD guideline, the highest suggested test concentration is 100 mg/L for a limit test.
Reference substance (positive control):
yes
Remarks:
Sodium chloride

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EL0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EL100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
mobility
Details on results:
- No immobilization was reported at a nominal loading rate of 100 mg/L.
- No immobilization observed in the control.
Results with reference substance (positive control):
EC50 (48 h) = 5.13 mg/L.

Applicant's summary and conclusion