4,4'-diaminostilbene-2,2'-disulphonic acid

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data from reliable peer reviewed data source

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

not specified

Analytical monitoring:

no

Details on sampling:

- Concentrations: Total 7 nominal concentrations of test chemical were taken for the study ranging from 10-320 mg/l.
- Sampling method: Stock solutions were prepared with DMSO (100 mg/l).

Vehicle:

yes

Remarks:

DMSO was used as a vehicle.

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: microalga

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Details on test conditions:

Controls with and without this vehicle were also setup during the study.

Reference substance (positive control):

not specified

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: p < 0.05

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

76 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Details on results:

The no observed effect concentration (NOEC) and median effective concentration (EC50) of test chemical was determined to be 32 and 76 mg/L, respectively.

Validity criteria fulfilled:

not specified

Conclusions:

Based on the effect on biomass of test chemical on test organism Selenastrum capricornutum ATCC 22662, the 72 hr NOEC and EC50 value was determined to be 32 and 76 mg/l, respectively.

Executive summary:

Short term toxicity study was conducted for 72 hrs for determining the effect of test chemical on aquatic algae Selenastrum capricornutum ATCC 22662. The study was performed in accordance with the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system. Total 7 nominal concentrations of test chemical were taken for the study ranging from 10-320 mg/l. Stock solutions were prepared with DMSO (100 mg/l). Controls with and without the vehicle were also setup during the study. On the basis of the effect of test chemical on biomass of the test organism Selenastrum capricornutum ATCC 22662, the 72 hr NOEC and EC50 value was determined to be 32 and 76 mg/l, respectively. Thus, test chemical was considered as toxic to aquatic algae and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.

Description of key information

Short term toxicity study was conducted for 72 hrs for determining the effect of test chemical on aquatic algae Selenastrum capricornutum ATCC 22662 (Secondary source, 1996). The study was performed in accordance with the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system. Total 7 nominal concentrations of test chemical were taken for the study ranging from 10-320 mg/l. Stock solutions were prepared with DMSO (100 mg/l). Controls with and without the vehicle were also setup during the study. On the basis of the effect of test chemical on biomass of the test organism Selenastrum capricornutum ATCC 22662, the 72 hr NOEC and EC50 value was determined to be 32 and 76 mg/l, respectively. Thus, test chemical was considered as toxic to aquatic algae and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

76 mg/L

EC10 or NOEC for freshwater algae:

32 mg/L

Additional information

Short term toxicity study was conducted for 72 hrs for determining the effect of test chemical on aquatic algae Selenastrum capricornutum ATCC 22662 (Secondary source, 1996). The study was performed in accordance with the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system. Total 7 nominal concentrations of test chemical were taken for the study ranging from 10-320 mg/l. Stock solutions were prepared with DMSO (100 mg/l). Controls with and without the vehicle were also setup during the study. On the basis of the effect of test chemical on biomass of the test organism Selenastrum capricornutum ATCC 22662, the 72 hr NOEC and EC50 value was determined to be 32 and 76 mg/l, respectively. Thus, test chemical was considered as toxic to aquatic algae and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.