N-Cocoyl L-Proline

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

The effect concentration was measured after 24h.

GLP compliance:

not specified

Remarks:

This test was performed for the evaluation of the substance as a cosmetic ingredient and not for REACH purposes.

Test material

Test material information

Constituent 1

Reference substance name:

1-(1-oxododecyl)-L-proline

Cas Number:

58725-39-6

Molecular formula:

C17H31NO3

IUPAC Name:

1-(1-oxododecyl)-L-proline

Constituent 2

Reference substance name:

1-(1-oxotetradecyl)-L-Proline

Cas Number:

86282-96-4

Molecular formula:

C19H35NO3

IUPAC Name:

1-(1-oxotetradecyl)-L-Proline

Constituent 3

Reference substance name:

1-(1-oxodecyl)-L-Proline

Cas Number:

86282-95-3

Molecular formula:

C15H27NO3

IUPAC Name:

1-(1-oxodecyl)-L-Proline

Constituent 4

Reference substance name:

1-(1-oxooctyl)-L-Proline

Cas Number:

86282-94-2

Molecular formula:

C13H23NO3

IUPAC Name:

1-(1-oxooctyl)-L-Proline

Constituent 5

Reference substance name:

1(1-oxohexadecyl)-L-proline

Cas Number:

59441-32-6

Molecular formula:

C21H39NO3

IUPAC Name:

1(1-oxohexadecyl)-L-proline

Constituent 6

Reference substance name:

Dodecanoylprolyl-Lproline

IUPAC Name:

Dodecanoylprolyl-Lproline

Constituent 7

Reference substance name:

Tetradecanoylprolyl-L-proline

IUPAC Name:

Tetradecanoylprolyl-L-proline

Constituent 8

Reference substance name:

Palmitoylprolyl-L-proline

IUPAC Name:

Palmitoylprolyl-L-proline

Constituent 9

Reference substance name:

1-(1-oxooctadecyl)-L-proline

Molecular formula:

C23H43NO3

IUPAC Name:

1-(1-oxooctadecyl)-L-proline

Constituent 10

Reference substance name:

potassium;chloride

Cas Number:

7447-40-7

Molecular formula:

KCl

IUPAC Name:

potassium;chloride

Constituent 11

Reference substance name:

Reference substance 001

Cas Number:

1310-58-3

Molecular formula:

KOH

Constituent 12

Reference substance name:

Tripotassium phosphate

Cas Number:

7778-53-2

IUPAC Name:

Tripotassium phosphate

impurity 1

Reference substance name:

Unknown impurities

IUPAC Name:

Unknown impurities

Specific details on test material used for the study:

Cocoyl proline is produced directly in water solution. In this test, cocoyl proline was tested in the commercialized form, so in aqueous solution.

This choice was taken considering that for measuring toxicity on aquatic invertebrates, solids need to be dissolved in water before testing. Furthermore, it provides a better understanding on the properties of the substance in the form that will be in contact with final users

Water percentage in the sample varies between 30 and 50%.

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution has been prepared dissolving a known amount of the sample in a specified volume of
dilution water (1 g/L). The test has been carried out starting from the concentration of 100 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The method requires Daphnia magna grown in laboratory under controlled conditions, while organisms (Ephippia) from specialized suppliers have been used in immobilized conditions and let open (about 72-80 hours, 6000 lux, 20-22°C, about 72-80) for the execution of the test. The crustaceans used for the test were not more than 24 hours old.

Study design

Test type:

static

Water media type:

not specified

Total exposure duration:

24 h

Test conditions

Hardness:

250 mg/L +/- 25 mg/L (on CaCO3)

Test temperature:

20-22°C

pH:

7,8 +/- 0,2

Dissolved oxygen:

7 mg/L

Conductivity:

10 μS/cm maximum

Nominal and measured concentrations:

A stock solution has been prepared dissolving a known amount of the sample in a specified volume of dilution water (1 g/L). The test has been carried out starting from the concentration of 100 mg/l. These were nominal concentrations.

Results and discussion

Effect concentrations

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

39.3 mg/L

Basis for effect:

mobility

Applicant's summary and conclusion

Conclusions:

On the basis of the assay, cocoyl proline showed effects on motility for aquatic invertebrates, in particular the endpoint was assessed at 39,3 mg/L (EC50 after 24h).