Reaction products of ammonium halide with phosphorus polyhalide, partially hydrolysed

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 11 - October 6, 1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Reference

Name:

Unnamed

Type:

Constituent

Test material form:

liquid

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

S9 liver microsomal fraction from male Sprague Dawley rats

Test concentrations with justification for top dose:

A preliminary toxicity test was performed on strain TA98 without metabolic activation. The following concentrations were tested : 0. 005 - 0. 01 -0.05 - 0.1 - 0.5 - 1 - 5 mg/plate. As the concentration of 5 mg/plate
was very toxic and 1 mg/plate affected the bacterial lawn, the following range was adopted for the definitive study : 0. 01 - 0. 05 - 0.1 - 0. 5 - 1 mg/plate.

Vehicle / solvent:

Acetone

Controls

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

9-aminoacridine

2-nitrofluorene

ethylmethanesulphonate

methylmethanesulfonate

Results and discussion

Test results

Test results 1

Key result

Species / strain:

S. typhimurium, other: TA 100, TA 1535, TA1537, TA1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Key result

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

Values obtained in the presence of test article was greater than twice the value obtained with the solvent control on strain TA 98.

Applicant's summary and conclusion

Conclusions:

PNCl2 is mutagenic on strain TA 98, with and without metabolic activation, not mutagenic on the others strains.
The mutagenic potency of test compound decreases in presence of metabolic activation.