Benzoic acid, 5-methyl-2-(2H-1,2,3-triazol-2-yl)-

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Ames test, but without full study report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2011

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

5-methyl-2-(2H-1,2,3-triazol-2-yl)benzoic acid

EC Number:

689-732-5

Cas Number:

956317-36-5

Molecular formula:

C10H9N3O2

IUPAC Name:

5-methyl-2-(2H-1,2,3-triazol-2-yl)benzoic acid

Details on test material:

Purity > 98 %

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

not specified

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A pKM 101

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

metaboloic activation by liver extract (S-9) from rats treated with xenobiotics

Test concentrations with justification for top dose:

100 to 5000 µg / plate

Vehicle / solvent:

N / A

Controls

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Details on test system and experimental conditions:

The test article was evaluated in an exploratory microbial mutation assay over a concentration range of 100 to 5000 µg / plate. Salmonella typhimurium test strains TA1535, TA97a, TA98, and TA100 and Escherichia coli test strain WP2 uvrA pKM101 were used, with and without metabolic activation (S-9 liver extract).

Evaluation criteria:

Positive result taken to be a 2-fold or greater increase in revertants relative to the solvent control.

Results and discussion

Test results

Test results 1

Species / strain:

other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Untreated negative controls validity:

valid

True negative controls validity:

not examined

Positive controls validity:

valid

Test results 2

Species / strain:

E. coli WP2 uvr A pKM 101

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Additional information on results:

The results indicated that the test article, up to the highest tested concentration, did not produce any 2-fold or greater increases in revertants relative to the solvent control in any of the test strains, either with or without metabolic activation. The positive control, 2-aminoanthracene (2AA) produced the expected increases in revertants in all test strains in the presence of metabolic activation. No precipitate was noted on the plates at any concentration tested. No inhibition of bacterial lawn growth was noted at any concentration tested. Slight inhibition of revertant growth was noted in TA98 at 5000 µg / plate with metabolic activation.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The test article was considered not to be mutagenic in this test system.

Executive summary:

The test article was evaluated in an exploratory microbial mutation assay over a concentration range of 100 to 5000µg / plate.Salmonella typhimurium test strains TA1535, TA97a, TA98, and TA100 and Escherichia colitest strain WP2 uvrA pKM101 were used, with and without metabolic activation (S-9 liver extract).

The results indicated that the test article, up to the highest tested concentration, did not produce any 2 -fold or greater increases in revertants relative to the solvent control in any of the test strains, either with or without metabolic activation. The positive control, 2 -aminoanthracene (2AA) produced the expected increases in revertants in all test strains in the presence of metabolic activation. No precipitate was noted on the plates at any concentration tested. No inhibition of bacterial lawn growth was noted at any concentration tested. Slight inhibition of revertant growth was noted in TA98 at 5000µg / plate with metabolic activation. In conclusion, the test article was considered not to be mutagenic in this test system.