Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2019.03.12~2019.05.20
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019
Report date:
2019

Materials and methods

Test guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Trimethyloctylammonium methyl sulphate
EC Number:
265-332-2
EC Name:
Trimethyloctylammonium methyl sulphate
Cas Number:
65059-42-9
Molecular formula:
C11H26N.CH3O4S
IUPAC Name:
trimethyl(octyl)azanium methyl sulfate

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Test conditions

Test temperature:
(21 - 24)℃

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
>= 12.83 - <= 14.97 mg/L
Nominal / measured:
nominal
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 1.12 mg/L
Nominal / measured:
nominal
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
ca. 3.54 mg/L
Nominal / measured:
nominal

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 72 h-EC50 of Trimethyloctylammonium methyl sulphate for freshwater alga ,Pseudokirchneriella subcapitata, is as follows. EC50, NOEC, and LOEC is expressed in measured concentration.

Inhibition rate on the average specific growth rate,
- 72 h-ErC50 : 13.86 mg/L {95 % confidence interval : (12.83 - 14.97) mg/L}
- 72 h-NOEC : 1.12 mg/L
- 72 h-LOEC : 3.54 mg/L (Annex 6, 7).

Inhibition rate on the yield,
- 72 h-ErC50 : 4.22 mg/L {95% confidence interval : (4.00 - 4.44) mg/L}
- 72 h-NOEC : 0.42 mg/L
- 72 h-LOEC : 1.12 mg/L (Annex 8, 9).

No factors influencing the test occurred during the study.