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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: screening test, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1979
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: In house Ciba Geigy Methode A-15
Version / remarks:
Comparable to internationally accepted guidelines
Deviations:
no
GLP compliance:
no
Specific details on test material used for the study:
None
Oxygen conditions:
aerobic
Inoculum or test system:
not specified
Details on inoculum:
None
Initial conc.:
500 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
None
Preliminary study:
None
Test performance:
None
Remarks on result:
not measured/tested
Details on results:
None
Parameter:
BOD5
Value:
0 mg O2/g test mat.
Remarks on result:
other: concentration 500 mg/L
Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
BOD5 of FAT 11127/B was found to be 0 mg O2/g.
Executive summary:

BOD5 of FAT 11127/B was determined according to in house Ciba Geigy Methode A.15, which is comparable to internationally accepted guidelines. Based on these investigations, the BOD5 of test substance was found to be 0 mg O2/g.

Considering the COD value determined with the test substance which is 466 mg O2/g, the resulting BOD5/COD quotient is also zero, indicating that the test substance is unlikely to be biodegradable.

Endpoint:
biodegradation in water: screening test, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1979
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: In house Ciba Geigy Methode A-16
Version / remarks:
Comparable to internationally accepted guidelines
Deviations:
no
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
not specified
Initial conc.:
20 mg/L
Based on:
test mat.
Details on study design:
None
Remarks on result:
not measured/tested
Key result
Parameter:
COD
Value:
466 mg O2/g test mat.
Remarks on result:
other: concentration 20 mg/L
Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
COD of FAT 11127/B was found to be 466 mg O2/g.
Executive summary:

COD of FAT 11127/B was determined according to in house Ciba Geigy Methode A.16. Based on the findings of the study, the COD of test substance was found to be 466 mg O2/g.

Considering the BOD5 value determined with the test substance which is zero, the resulting BOD5/COD quotient is also zero, indicating that the test substance is unlikely to be biodegradable.

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
From 02 Feb, 1994 to 18 April, 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: EEC-Directive, L133 (pp. 99-105) Volume 31, May 88
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: internal method - SAV:13 000 02
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Code number : FAT· 11127/C
Batch-Nr. : 9309001
Purity : ca. 80 %
Appearance : solid
Solubility : >2 g/l (in water)
Storage : room temperature
Expiry date : 06/99
Oxygen conditions:
not specified
Inoculum or test system:
other: Sludge of a biological sewage treatment plant (ARA-Therwil) collected on 08.02.94
Duration of test (contact time):
28 d
Initial conc.:
150 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
Experimental conditions:
Water / Nutrient: according to the specification of the EEC L133 (pp. 99-105) Volume 31 resp. OECD Method 302B
Temperature: 22.0 ± 3°C (room temperature)
Test duration : 28 days
Lighting : Indirect daylight
Estimation technique : TOC/DOC Analyzer/SHIMADZU TOC-5000
Reference substance:
diethylene glycol
Remarks:
FAT-90'019/E (Fluka No. 32170]
Key result
Parameter:
% degradation (DOC removal)
Value:
32.9
Sampling time:
28 d
Details on results:
Test substance : Adsorption after 3 hours: 23.6 %
Results with reference substance:
The bioelimination, corrected by the blank control and measured as DOC (mg/I) was 99.4 % on 14th day.
Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable, fulfilling specific criteria
Remarks:
inherent, primary biodegradability (>20 % DOC removal)
Conclusions:
The bioelimination of the test substance FAT 11127/C after 28 days was found to be 32.9 %.
Executive summary:

The inherent biodegradability of FAT 11127/C was determined in a 28 days Zahn-Wellens- Test according to OECD Guideline 302B and EEC-Directive,L133.

The test substance and reference substance (Diethylenglycol) were tested at concentration of 150 mg/I DOC.

The bioelimination of the reference substance after 14 days was found to be 99.4 %.

The bioelimination of the test substance FAT 11127/C after 28 days was found to be 32.9 %. Hence, it can be concluded that under the test conditions, FAT 11127/C demonstrated inherent, primary biodegradability.

Description of key information

Direct Blue 094 is not expected to be readily biodegradable, but to have inherent, primary biodegradation potential.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable, fulfilling specific criteria
Type of water:
freshwater

Additional information

Direct Blue 094 has been investigated for biodegradation in different studies evaluating Biological Oxygen Demand (BOD5), Chemical Oxygen Demand (COD) and inherent biodegradability.

The BOD5 and COD for FAT 11127/A were found to be 0 mg O2/g, and 332 mg O2/g respectively. Similarly, BOD5 and COD of FAT 11127/B was found to be 0 mg O2/g, and 466 mg O2/g, respectively. BOD5/COD ratios for both FAT 11127/A and 1127/B are zero, indicating that ready biodegradation cannot be expected.

FAT 11127/A was evaluated for biological elimination in a study conducted using test method FC 2.463, 1-7.1973. The bioelimination of the test substance was 10 %, hence it can considered to be not inherently biodegradable.

FAT 11127/C was found to bioeliminate 32.9 % after 28 days in a Zahn-Wellens-Test conducted according to OECD Guideline 302B and EEC-Directive, L133.

Hence, it can be concluded that under the test conditions, FAT 11127/C demonstrated inherent, primary biodegradability.

 

Based on the above information, Direct Blue 094 can be expected to have some inherent biodegradation potential, however, it is not expected to be readily biodegradable.