Diheptyl succinate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Diheptyl succinate

EC Number:

239-996-9

EC Name:

Diheptyl succinate

Cas Number:

15872-89-6

Molecular formula:

C18H34O4

IUPAC Name:

1,4-diheptyl butanedioate

Test material form:

liquid

Details on test material:

LOT# BH9326
CAS No. 15872-89-6
sample date: Aug/06/2012
Acid Value, mg KOH/g 0.10 Max.
Kinematic Visc.@25°C, cSt 7.0 to 9.0

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

DILUTION WATER: Fortified well water
pH: 8.1
Conductivity: 520 μS/cm
Total hardness as CaCO3: 160 mg/L
Total alkalinity as CaCO3: 90 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna,
Age: < 24 hours old at exposure initiation
Source: Smithers Viscient culture facility

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

Total hardness as CaCO3: 160 mg/L

Test temperature:

temperature range of 19 to 21 ºC

pH:

8.1

Salinity:

Total alkalinity as CaCO3: 90 mg/L

Conductivity:

Conductivity: 520 μS/cm

Nominal and measured concentrations:

nominal test conc. 6.3, 13, 25, 50 and 100 mg/L

Details on test conditions:

48-hour duration, temperature range of 19 to 21 ºC, photoperiod of 16 hours light and 8 hours darkness, light intensity range of 32 to 85 footcandles (340 to 910 lux)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Based on nominal concentrations of diheptyl succinate, the 48−hour EC50 value was empirically estimated to be > 100 mg/L, the highest nominal concentration tested. The No-Observed-Effect Concentration (NOEC) was determined to be 100 mg/L. The highest concentration producing 0% immobilization was 100 mg/L. The lowest concentration producing 100% immobilization was > 100 mg/L.
Additional testing to further define the EC50 value was not performed since the highest nominal concentration tested (100 mg/L) represents the highest concentration required by the OECD testing guidelines.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations of diheptyl succinate, the 48−hour EC50 value was empirically estimated to be > 100 mg/L, the highest nominal concentration tested. The No-Observed-Effect Concentration (NOEC) was determined to be 100 mg/L. The highest concentration producing 0% immobilization was 100 mg/L. The lowest concentration producing 100% immobilization was > 100 mg/L.
Additional testing to further define the EC50 value was not performed since the highest nominal concentration tested (100 mg/L) represents the highest concentration required by the OECD testing guidelines.