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EC number: 418-400-2 | CAS number: 1031-15-8 METHYL-TPP-CHLORID; MTPP-CHLORID
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jan 25 - March 24, 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Version / remarks:
- July 17, 1992
- Deviations:
- no
- GLP compliance:
- yes
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- The available study was performed in 1994, the LLNA test was was not the standard in vivo method by this time. Therefore a GPMT test was performed.
Test material
- Reference substance name:
- Methyltriphenylphosphonium chloride
- EC Number:
- 418-400-2
- EC Name:
- Methyltriphenylphosphonium chloride
- Cas Number:
- 1031-15-8
- Molecular formula:
- C19H18ClP
- IUPAC Name:
- methyltriphenylphosphanium chloride
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Sex:
- male/female
- Details on test animals and environmental conditions:
- Strain: Pirbright white
Substrain: HsdlWin: DH
Source: Firma Harlan Winkelmann, Versuchstierzucht, Gartenstr.27 33178 Borchen
Acclimatization period: 7 days (range finding), 23 days (main test)
Animal selection: random
Animal identification: with colored markings; cage labelled with sex, date of study initiation, project no.
Weight range: male: 310 - 574 g / female: 275 - 597 g
Housing:
collective housing up to a maximum of 5 animals per cage (Makrolon@ type IV)
illumination:
artificial lighting (120 lux) from 7.00 a.m. -7.00 p.m.
Temperature:
22 +/- 3°C
Relative Humidity:
30-70%
Measurement:
twice daily
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal
- Vehicle:
- water
- Concentration / amount:
- 5 %
- Day(s)/duration:
- 7 days
- Adequacy of induction:
- non-irritant substance, but skin pre-treated with 10% SDS
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: vaseline
- Concentration / amount:
- 50% (w/w)
- Day(s)/duration:
- 14 days
- Adequacy of induction:
- not specified
Challenge
- No.:
- #1
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: vaseline
- Concentration / amount:
- 50%
- Day(s)/duration:
- 1 day
- Adequacy of challenge:
- highest non-irritant concentration
- No. of animals per dose:
- 20 animals
- Details on study design:
- Prestudy:
Intradermal Injection
The test article was diluted with aqua ad iniect. and Freund's complete adjuvant (PCA; Sigma, 80241 Deisenhofen) to give a final concentration of 5 %. Two animals were employed, skin reactions being recorded 48 h after treatment.
Dermal Application
The test article was incorporated in vaseline to provide a final concentration of 50 % (w/w). A closed patch exposure was effected by means of an occlusive bandage using Hill-Top Chambers (Hill Top, Cincinnati, USA) and non-irritating tape Elastoplast@ (Beiersdorf AG, Hamburg), which enveloped the whole of the animal's trunk. Two animals were employed and skin reactions were recorded 48 h post applicationem.
Main study:
The main study was performed on 20 test and 10 control animals. On the basis of the results of the range finding the concentration of 5 % of the test article was considered to be suitable for intradermal injection and the concentration of 50 % for dermal application.
Induction Procedure: First stage - an area of 4 x 6 cm over the shoulders was clipped short with electric clippers and cleaned with 70 % (v/v) ethanol. lbree pairs of intradermal injections were then made symmetrically in two rows on either side of the spine.
Test group:
1. 0.1ml FCA 50 % (w/w) diluted inaqua ad iniect.
2. 0.1 ml test article diluted in aqua ad iniectabilia (final concentration: 5 %)
3. 0.1 ml test article diluted in FCNaqua ad iniect. (final concentration: 5 %)
Control group:
1. 0.1 ml FCA 50% (w/w) diluted in aqua ad iniect.
2. 0.1 ml aqua ad iniectabilia
3.0.1 ml aqua ad iniectabilia 50% (w/w) diluted in FCA
Second stage - 7 days after the intradermal injections, dermal application was initiated. Because the test article was non-irritating at the highest permissible concentration in the pilot study, the area was redipped and pretreated with 10 % sodium lauryl sulfate (SLS) in vaseline 24 h before application of the test article at a concentration of 50 % in vaseline. The test article was spread in a thick layer [to saturation] over a 4 x 5 cm patch (filter paper). The latter was firmly secured over the previous injection sites by an occlusive dressing for 48 h. Control animals received a patch loaded with the vehicle alone.
Challenge procedure
Both control and test animals were subjected to a challenge exposure 14 days after the second stage of induction. The challenge test was performed on a 5 x 5 cm clipped area on each flank. The maximal non-irritating concentration of the test article (50 % in vaseline) was applied to the left flank and vaseline to the right (0.5 g in each case) using the patch technique described under 4.3.2. The duration of exposure was 24 h under an occlusive dressing. 24 and 48 h after patch removal, the treated skin areas were evaluated on a numerical scale according to Draize. - Positive control substance(s):
- yes
Results and discussion
- Positive control results:
- The reaction to the positive control substances 2,4 dinitrochlorobenzene (extreme sensitizer) and benzocaine (moderate sensitizer) is tested periodically. The last test with an acceptable level of response to each of these substances was performed in November, 1993.
In vivo (non-LLNA)
Resultsopen allclose all
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 50 % in vaseline
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- No skin reactions were observed. Two test animals died within the induction phase on day 8 (no. 6) or day 11 (no. 14). The cause of death is not clear.
- Remarks on result:
- no indication of skin sensitisation
- Key result
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 50 % in vaseline
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- No skin reactions were observed. Two test animals died within the induction phase on day 8 (no. 6) or day 11 (no. 14). The cause of death is not clear.
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 50% in vaseline
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 50% in vaseline
- No. with + reactions:
- 0
- Total no. in group:
- 10
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the conditions of the study, the test item is considered to be a dermal sensitiser.
- Executive summary:
The potential skin sensitizing properties of the test item were assessed in the guinea pig maximization test using 20 test and 10 control animals. Following induction exposure to the
test article or the vehicle, the animals were subjected two weeks later to a challenge exposure with the test article. The treated skin areas were evaluated 24 and 48 h after the end of the exposure period. According to the OECD guideline for testing of chemicals (OECD 406, July 17, 1992), since no animal showed an allergic response, the test article applied as a 50 % preparation, may be classified as a "non-sensitizer".
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