2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-3-[2-[3-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-6-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-, sodium salt (1:5)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From October 24, 2017 to December 18, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: National Chiayi University Freshwater Biological Resource Center

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Nominal and measured concentrations:

For range-finding test: 0.16, 0.080, 4.00, 20.00 and 100.00 mg/L
For limit test: 100.00 mg/L

Details on test conditions:

- Test volume: 30 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light: 8-hr dark

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

The results of the range-finding test showed that EC₅₀was greater than 100.00 mg/L.

In limit test, the results showed below:

 

Table 1. The numbers of immobilised daphnids for the limit test on day 1 and 2

Goup (mg/L)		Day	Numbers of immobilized activity	Numbers of discoloration	Numbers of trapping at surface of water
Control group	0.00	Day 1	0	0	0
Test group	100.00		0	0	0
Control group	0.00	Day 2	0	0	0
Test group	100.00		0	0	0

Table 2. The percentages immobilized and EC₅₀for the limit test

Group (mg/L)		Percentages immobilised (%)		EC50 (mg/L)
		Day 1	Day 2
Control group	0.00	0.00	0.00	−
Test group	100.00	0.00	0.00	>100.00

Table 3. Dissolved oxygen and pH value for the limit test

Group (mg/L)		Days	Dissolved oxygen^a (mg/L)	pH value^b
Control group	0.00	Day 0	8.76 ± 0.01	7.55 ± 0.05
		Day 1 (Before water-renewal)	8.69 ± 0.10	7.59 ± 0.06
		Day 1 (After water-renewal)	8.75 ± 0.01	7.65 ± 0.06
		Day 2	8.80 ± 0.02	7.54 ± 0.10
Test group	100.00	Day 0	8.71 ± 0.04	7.97 ± 0.04
		Day 1 (Before water-renewal)	8.78 ± 0.04	8.01 ± 0.03
		Day 1 (After water-renewal)	8.62 ± 0.09	7.79 ± 0.01
		Day 2	8.77 ± 0.01	7.76 ± 0.06

^a Dissolved oxygen concentrations showed as mean ± standard deviation.

^b pH value showed as mean ± standard deviation.

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 202 test method, the 48-hr EC50 of CR SB37 was >100 mg/L.

Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-170900012001 which is based on the SOP for the OECD 202 (SOPE-505) and OECD 202 (OECD, 2004). The results of this OECD 202 test for CR SB37 show that all validity criteria were met. Under the conditions of this test, the 48-hr EC50 of R SB37 was >100 mg/L.

Description of key information

The 48-hr EC50 of CR SB37 was >100 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

In both range-finding and limit test, not more than 10 % of the Daphnia were immobilised in the control and the dissolved oxygen concentration at the end of the test was ≥3 mg O₂/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. Under the conditions of this test, the 48-hr EC50 of CR SB37 was >100 mg/L.