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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From October 24, 2017 to December 18, 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: National Chiayi University Freshwater Biological Resource Center
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Nominal and measured concentrations:
For range-finding test: 0.16, 0.080, 4.00, 20.00 and 100.00 mg/L
For limit test: 100.00 mg/L
Details on test conditions:
- Test volume: 30 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light: 8-hr dark
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

The results of the range-finding test showed that EC50was greater than 100.00 mg/L.

In limit test, the results showed below:

 

Table 1. The numbers of immobilised daphnids for the limit test on day 1 and 2

Goup

(mg/L)

Day

Numbers of immobilized activity

Numbers of discoloration

Numbers of trapping at surface of water

Control group

0.00

Day 1

0

0

0

Test group

100.00

0

0

0

Control group

0.00

Day 2

0

0

0

Test group

100.00

0

0

0

Table 2. The percentages immobilized and EC50for the limit test

Group

(mg/L)

Percentages immobilised (%)

EC50

(mg/L)

Day 1

Day 2

Control group

0.00

0.00

0.00

Test group

100.00

0.00

0.00

>100.00

Table 3. Dissolved oxygen and pH value for the limit test

Group

(mg/L)

Days

Dissolved oxygena

(mg/L)

pH valueb

Control group

0.00

Day 0

8.76 ± 0.01

7.55 ± 0.05

Day 1 (Before water-renewal)

8.69 ± 0.10

7.59 ± 0.06

Day 1 (After water-renewal)

8.75 ± 0.01

7.65 ± 0.06

Day 2

8.80 ± 0.02

7.54 ± 0.10

Test group

100.00

Day 0

8.71 ± 0.04

7.97 ± 0.04

Day 1 (Before water-renewal)

8.78 ± 0.04

8.01 ± 0.03

Day 1 (After water-renewal)

8.62 ± 0.09

7.79 ± 0.01

Day 2

8.77 ± 0.01

7.76 ± 0.06

a Dissolved oxygen concentrations showed as mean ± standard deviation.

b pH value showed as mean ± standard deviation.

Validity criteria fulfilled:
yes
Conclusions:
According to OECD 202 test method, the 48-hr EC50 of CR SB37 was >100 mg/L.
Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-170900012001 which is based on the SOP for the OECD 202 (SOPE-505) and OECD 202 (OECD, 2004). The results of this OECD 202 test for CR SB37 show that all validity criteria were met. Under the conditions of this test, the 48-hr EC50 of R SB37 was >100 mg/L.

Description of key information

The 48-hr EC50 of CR SB37 was >100 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

In both range-finding and limit test, not more than 10 % of the Daphnia were immobilised in the control and the dissolved oxygen concentration at the end of the test was ≥3 mg O2/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. Under the conditions of this test, the 48-hr EC50 of CR SB37 was >100 mg/L.