5-hydroxyiminobarbituric acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

following GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

adopted 17 July 1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

5-hydroxyiminobarbituric acid

EC Number:

201-741-4

EC Name:

5-hydroxyiminobarbituric acid

Cas Number:

87-39-8

Molecular formula:

C4H3N3O4

IUPAC Name:

5-(hydroxyimino)-1,3-diazinane-2,4,6-trione

Test material form:

solid: particulate/powder

Details on test material:

Lot/batch No.: 201713807

Specific details on test material used for the study:

Name: Violuric Acid
CAS Number: 87-39-8
Molecular Formula: C4H3N3O4
Molecular Weight: 157.08 g/mol
Physical State: Powder (solid)
Appearance: Off white
Batch Number: DFB083-172
Purity: 98.2 %
Date sample received: 20 August 2015

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

A sample of activated sludge was obtained from Worlingworth sewage treatment works (Suffolk, UK), which treats predominantly domestic waste.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

25 other: Violuric acid (25 mg O2/500 mL)

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Remarks on result:

other:

Remarks:

There was no oxygen consumption in mixtures containing Violuric acid throughout the test

BOD5 / COD results

Results with reference substance:

The blank-corrected oxygen demanded by the culture containing the reference substance had achieved 16.03 mg O2/500 mL or 64% of the ThOD (25 mg O2/500 mL) after 3 days of incubation.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

There was no oxygen consumption that could be related to the biodegradation Violuric acid in the test. Substances are considered to be readily biodegradable in this type of test if the oxygen consumption is equal to, or greater than 60% of the ThOD of the test mixtures within ten days of the consumption achieving 10%. Therefore, Violuric acid is considered to be not readily biodegradable under the conditions of this test.

Executive summary:

The biodegradation of Violuric acid was studied according to the OECD Guideline no. 301F and following GLP.

There was no oxygen consumption that could be related to the biodegradation Violuric acid in duration of the test. Substances are considered to be readily biodegradable in this type of test if oxygen consumption is equal to or greater than 60% of the ThOD of the test mixtures within ten days of the consumption achieving 10%.  Therefore, Violuric acid is considered to be not readily biodegradable under the conditions of this test.