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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
A saturated solution of Donor C20 was prepared by mechanical stirring for 20 hours followed by 4h standing.
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20°C
Nominal and measured concentrations:
Donor C20 was tested at its aqueous solubility and at its 10, 18, 32, and 56 volume percent dilutions.
The acutal concentration of Donor C20 was determined by chemical analysis
Details on test conditions:
The test was carried out under 16h light/8h dark regime. The test was performed in 150 ml all-glass beakers, each containing 100 ml of control or test medium. Four beakers containing 5 daphnids each were used for the control or test medium.
The control and test media were not aerated or replaced. The daphnids were not fed. The pH and oxygen concentration were measured at the beginning and at the end of the test.
The test vessels were checked for mobility of the test animals after 24 hours and at the end of the test. At the same time the condition of the mobile animals was compared with that of control animals.
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
>= 7.9 mg/L
Nominal / measured:
estimated
Basis for effect:
mobility
Remarks on result:
other: Above the limit of aqueous solubility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
7.9 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
mobility
Remarks on result:
other: Limit of aqueous solubility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
4.42 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
behaviour
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
7.9 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
behaviour
Details on results:
An effect was observed at the highest dose tested with the animals showing a slow and disturbed swimming behaviour. This led to setting the LOEC at the highest dose tested (100% aqueous solubility)
Validity criteria fulfilled:
yes
Conclusions:
With respect to mobility, Donor C20 is not acutely toxic to Daphnia magna. With regards to the condition observed at the highest achievable water saturation, slow and disturbed swimming, establishing that dose (7.9 mg/l) as the LOEC with the NOEC at 56% of the maximum solubility or 4.42 mg/l.

Description of key information

In a GLP-compliant, OECD guideline study, changes in swimming behaviour were observed at the highest concentration tested, however, no effects on mobility was observed at the limit of aqueous solubility (7.9 mg/l).

Key value for chemical safety assessment

Additional information

It is not appropriate to include an EC50 value for the chemical safety assessment above as this is determined to be greater than the limit of aqueous solubility.