Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
10.4.2018-14.4.2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2012
Qualifier:
according to guideline
Guideline:
other: OECD series on testing and assessment No 23 Guidance document on aquatic toxicity testing of difficult substances and mixtures.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Lawsonia inermis, ext.
EC Number:
284-854-1
EC Name:
Lawsonia inermis, ext.
Cas Number:
84988-66-9
Molecular formula:
na
IUPAC Name:
Lawsonia inermis, ext.
Test material form:
solid: particulate/powder
Details on test material:
Batch number:ONH/17/I/01

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified
Details on test solutions:
Fresh extraction of the test concentration was prepared at room temperature in normal lighting. Teh test concentration was stirred at a speed that created a small vortex on the surface for 24 hours in a covered glass beaker. After being allowed to settle for 1 hour, the volume was filtered in two steps.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The Daphnia magna culture is a Clone-A originating from the Norwegian Institute of Water Research (NIVA), Norway. The Clone-A has been indentified by genotyping.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
20±1°C
pH:
7,7-8,0
Dissolved oxygen:
5,7-8,6 mg/l

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 - <= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The immobilisation of the Daphnia magna at the concentration 100 mg/l was 0% after 48 hours and therefor the NOEC-valu is determinded to be 100 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes