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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Remarks:
Mobility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Frm January 19, 2016 to January 25, 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Batch no.: 145
Purity: 37.6% in water
Solubility: soluble in water
Analytical monitoring:
yes
Remarks:
Measurement of analytical total organic carbon (TOC) concentrations with a TOC analyser
Details on sampling:
- Samples, properly diluted with deionised water with low TOC contents, have been analysed by means of an integrated sampling system directly from vials containing 40 ml volume. For each sample under test the corresponding blank is analysed.
- Calculations:
% stability: (TOC test subst. 48h - TOC control 48h)/(TOC test subst. 0h - TOC control 0h). Samples are considered stable if the % stability is between 80 and 120% of the value.
- The samplings were stable throughout the test period.
Vehicle:
no
Details on test solutions:
Range finding test: 0.1 - 1000 mg/L
Definitive test: 100 mg/L
Test organisms (species):
Daphnia magna
Details on test organisms:
Age: 6 - 24 hours
Origine: Breeder in Eurofins Biolab Test Facility
Feeding: green alga
Number of animals: 20/group
Number of replications: 4/group
Test type:
static
Water media type:
other: deionized water + dissolved salts (CaCl2.H2O, MgSO4.7H2O, KCl, NaHCO3)
Limit test:
yes
Total exposure duration:
48 h
Hardness:
250 mg CaCO3/L
Test temperature:
20.0 +/- 0.1°C
pH:
7.44 - 7.85
Dissolved oxygen:
5.79 - 6.99 mg/L
Nominal and measured concentrations:
Range finding test: 0.1 - 1000 mg/L
Definitive test: 1000 mg/L
Details on test conditions:
Photoperiod: 16 hours of light, 8 hours of darkness
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
act. ingr.
Basis for effect:
mobility
Details on results:
No immobilised Daphnia were observed at this dose level. No immobilised animals were reported in the control group.
Results with reference substance (positive control):
-
Validity criteria fulfilled:
yes
Conclusions:
Under the study conditions, the 48 h EC50 of the substance in Daphnia magna was determined to be >100 mg a.i./L.
Executive summary:

A study was conducted to determine the short-term toxicity of the substance to aquatic invertebrates according to OECD Guideline 202, in compliance with GLP. Based on a range-finding study, Daphnia magna were exposed to a concentration of 100 mg/L of the test substance for 48 h. Total organic carbon (TOC) concentrations were measured with a TOC analyser and the test substance was considered stable over the duration of the test. No immobilised Daphnia were observed at this dose level. No immobilised animals were reported in the control group. Under the study conditions, the 48 h EC50 of the substance in Daphnia magna was determined to be >100 mg a.i./L (Giarei, 2016).

Description of key information

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

A study was conducted to determine the short-term toxicity of the substance to aquatic invertebrates according to OECD Guideline 202, in compliance with GLP. Based on a range-finding study, Daphnia magna were exposed to a concentration of 100 mg/L of the test substance for 48 h. Total organic carbon (TOC) concentrations were measured with a TOC analyser and the test substance was considered stable over the duration of the test. No immobilised Daphnia were observed at this dose level. No immobilised animals were reported in the control group. Under the study conditions, the 48 h EC50 of the substance in Daphnia magna was determined to be >100 mg a.i./L (Giarei, 2016).