Reaction mass of N,N'-ethylenebis[N-methyl-2-[(1-oxo-9-octadecenyl)oxy]-N-[2-[(1-oxo-9-octadecenyl)oxy]propyl]propylammonium] dimethyl disulphate and [2-[bis(2-hydroxypropyl)amino]ethyl]bis(2-hydroxypropyl)(methyl)ammonium methyl sulphate, dioleate (ester)

Melting point / freezing point

Administrative data

Endpoint:

melting point/freezing point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01.02.2018 - 28.08.2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 102 (Melting point / Melting Range)

GLP compliance:

no

Type of method:

method to determine freezing temperature

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of N,N'-ethylenebis[N-methyl-2-[(1-oxo-9-octadecenyl)oxy]-N-[2-[(1-oxo-9-octadecenyl)oxy]propyl]propylammonium] dimethyl disulphate and [2-[bis(2-hydroxypropyl)amino]ethyl]bis(2-hydroxypropyl)(methyl)ammonium methyl sulphate, dioleate (ester)

EC Number:

916-222-4

Molecular formula:

C51H99N2O6.CH3O4S

IUPAC Name:

Reaction mass of N,N'-ethylenebis[N-methyl-2-[(1-oxo-9-octadecenyl)oxy]-N-[2-[(1-oxo-9-octadecenyl)oxy]propyl]propylammonium] dimethyl disulphate and [2-[bis(2-hydroxypropyl)amino]ethyl]bis(2-hydroxypropyl)(methyl)ammonium methyl sulphate, dioleate (ester)

Specific details on test material used for the study:

Name: AGENT 5088-127A
Lot No. Purity Manufactured date Retest date
5124-143-001 100% 20/12/2017 20/12/2020

Results and discussion

Melting / freezing point

Key result

Melting / freezing pt.:

-7.8 °C

Atm. press.:

1 atm

Decomposition:

no

Sublimation:

no

Applicant's summary and conclusion

Conclusions:

The results of the study indicate the test item to have started to melt at
-7.8°C wherein it forms a gel like substance. Further, at 22.6°C the test item forms a viscous thick liquid and at 47.7°C onwards the test item was less viscous and free flowing liquid.

Executive summary:

The test item, AGENT5088-127Ais UVCB substance and have constituents in an unknown and variable quantity. The test item is highly viscous thick liquid in nature at room temperature. As determination of melting point below ambient temperature was not feasible at the test facility, it was decided to first freeze the test item below zero degrees and then record the temperature (using a calibrated thermometer) at which the test item starts to melt.

About 2.0 g of test item was taken in a narrow test tube, stoppered and immersed in a beaker containing methanol such that the test tube is dipped in methanol slightly above the level of the test item in the test tube. This set up was kept in a deepfreezer maintained below -20°C a minimum of 50 hours so as to allow the test item to freeze and crystallize/solidify. After about
68 hours the beaker containing the test tube with the test item was taken out of the deep freezer and immediately the physical state of the test item was recorded which indicated the test item to have formed a solid like mass which was not flowing/moving. Later, the beaker was allowed thaw to room temperature and simultaneously, the temperature of the methanol in the beaker was monitored using a calibrated thermometer. At about -7.8°C, the physical state of the test item started to change from solid like mass to a gel like substance indicating the test item had started to melt at -7.8°C. After some more time when the temperature of methanol in the beaker reached 22.6°C, the test item had changed in to a viscous thick liquid indicating the test item had returned to its earlier/original physical state at room temperature. Further rise in temperature did not alter the physical state significantly until up to 47.7°C at which the test item was less viscous and free flowing liquid. At around 67.8°C, the test item was more free flowing.