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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
01.05.1995-17.05.1995
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
GLP compliance:
not specified
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Lot 408
- Purity: 95%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability under test conditions: stable
Analytical monitoring:
not specified
Details on sampling:
- Concentrations: 0, 0.5, 1.0, 50, 500, 5000 mg/L
Vehicle:
not specified
Details on test solutions:
not specified
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: ceriodaphnia dubia
- Strain/clone: not specified
- Source: not specified
- Age of parental stock (mean and range, SD): not specified
- Feeding during test: not specified
- Food type: not specified
- Amount: not specified
- Frequency: not specified

ACCLIMATION
- Acclimation period: not specified
- Acclimation conditions (same as test or not): same
- Type and amount of food: not specified
- Feeding frequency: not specified
- Health during acclimation (any mortality observed): not specified
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
Readings taken after 24h due to total mortality.
Hardness:
not specified
Test temperature:
not specified
pH:
between 7.2 and 8.6
Dissolved oxygen:
between 8.4 and 8.6 mg/L
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
0-5000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: not specified
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: not specified
- Aeration: not specified
- Type of flow-through (e.g. peristaltic or proportional diluter): not specified
- Renewal rate of test solution (frequency/flow rate): not specified
- No. of organisms per vessel: not specified
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified
- No. of vessels per vehicle control (replicates): not specified
- Biomass loading rate: not specified

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: not specified
- Total organic carbon: not speciifed
- Particulate matter: not specififed
- Metals: not specified
- Pesticides: not specified
- Chlorine: not specified
- Alkalinity: not specified
- Ca/mg ratio: not specified
- Conductivity: not specified
- Salinity: not specified
- Culture medium different from test medium: not specified
- Intervals of water quality measurement: not specified

OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: not specified
- Light intensity: not specifed
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
< 0.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: not specified
- Observations on body length and weight: not specified
- Other biological observations: not specified
- Mortality of control: not specified
- Other adverse effects control: not spcified
- Abnormal responses: not specified
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not specified
- Effect concentrations exceeding solubility of substance in test medium: not specified
Results with reference substance (positive control):
- Results with reference substance valid? not specified
- Relevant effect levels: not specified
- Limit test: not specified
- Dose-response test: not specified
- ECx: not specified
- Other: not specified
Reported statistics and error estimates:
not specified

Dissolved oxygen and pH:

At Test Initiation At Test Completion
pH Dissolved Oxygen (mg/L) pH Dissolved Oxygen (mg/L)
Replicate Replicate Replicate Replicate
Nominal concentration replicate (mg/L) A B A B A B A B
0 8.5 8.5 8.6 8.6 8.2 8.2 8.6 8.6
0.5 8.5 8.5 8.6 8.6 8.2 8.2 8.6 8.6
1 8.6 8.6 8.6 8.5 8.3 8.3 8.6 8.6
50 8.6 8.6 8.5 8.5 8.3 8.3 8.6 8.6
500 8.4 8.5 8.5 8.5 8.3 8.3 8.6 8.6
5000 7.2 7.2 8.6 8.6 7.7 7.7 8.4 8.4

Reading taken approximately 24 hours after exposure due to total mortatility.

Validity criteria fulfilled:
yes
Conclusions:
The test material exhibited extreme toxicity in a 48hour, unaerated, static acute test.
Executive summary:

The rangefinding study was designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. Nominal concentrations tested: 0, 0.5, 1.0, 50, 500, 5000 mg/L. The pH and dissolved oxygen readings have been taken approximately 24 hours after exposure due to total mortality.

Results:

Based on visual observations, all solutions were clear at the test start, except for 50mg/L which was slightly cloudy, white in color. Immobilities were 0, 100, 100, 100, 100 and 100% respectively. The 48 -hour EC50, based on nominal concentrations and immobility, was less than 0.5 mg/L. All water quality parameters were within acceptable limits.

The test substance exhibited extreme toxicity in a 48 -hour, unaerated, static avute test using Ceriodaphnia dubia.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
01.05.1995-17.05.1995
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
GLP compliance:
not specified
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Lot 408
- Purity: 95%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability under test conditions: stable
Analytical monitoring:
not specified
Details on sampling:
- Concentrations: 0, 0.5, 1.0, 50, 500, 5000 mg/L
Vehicle:
not specified
Details on test solutions:
not specified
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: daphnia magna
- Strain/clone: not specified
- Source: not specified
- Age of parental stock (mean and range, SD): not specified
- Feeding during test: not specified
- Food type: not specified
- Amount: not specified
- Frequency: not specified

ACCLIMATION
- Acclimation period: not specified
- Acclimation conditions (same as test or not): same
- Type and amount of food: not specified
- Feeding frequency: not specified
- Health during acclimation (any mortality observed): not specified
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
Reading taken after 24h exposure due to total mortality.
Hardness:
not specified
Test temperature:
not specified
pH:
7.0-8.3
Dissolved oxygen:
8.7-8.8
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
not specified
Details on test conditions:
TEST SYSTEM
- Test vessel: not specified
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: not specified
- Aeration: not specified
- Type of flow-through (e.g. peristaltic or proportional diluter): not specified
- Renewal rate of test solution (frequency/flow rate): not specified
- No. of organisms per vessel: not specified
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified
- No. of vessels per vehicle control (replicates): not specified
- Biomass loading rate: not specified

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: not specified
- Total organic carbon: not speciifed
- Particulate matter: not specififed
- Metals: not specified
- Pesticides: not specified
- Chlorine: not specified
- Alkalinity: not specified
- Ca/mg ratio: not specified
- Conductivity: not specified
- Salinity: not specified
- Culture medium different from test medium: not specified
- Intervals of water quality measurement: not specified

OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: not specified
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
< 0.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: not specified
- Observations on body length and weight: not specified
- Other biological observations: not specified
- Mortality of control: not specified
- Other adverse effects control: not spcified
- Abnormal responses: not specified
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not specified
- Effect concentrations exceeding solubility of substance in test medium: not specified
Results with reference substance (positive control):
- Results with reference substance valid? not specified
- Relevant effect levels: not specified
- Limit test: not specified
- Dose-response test: not specified
- ECx: not specified
- Other: not specified

Dissolved Oxygen and pH:

At Test Initiation At Test Completion
pH Dissolved Oxygen (mg/L) pH Dissolved Oxygen (mg/L)
Replicate Replicate Replicate Replicate
Nominal concentration replicate (mg/L) A B A B A B A B
0 8.3 8.3 8.7 8.7 8.3 8.3 8.7 8.7
0.5 8.3 8.3 8.7 8.7 8.3 8.3 8.7 8.7
1 8.3 8.3 8.7 8.7 8.3 8.3 8.7 8.7
50 8 8 8.7 8.7 8 8 8.7 8.7
500 7.5 7.5 8.7 8.7 7.8 7.8 8.7 8.7
5000 7 7 8.7 8.7 7.4 7.4 8.8 8.8

Reading taken approximately 24 hours after exposure due to total mortality.

Validity criteria fulfilled:
yes
Conclusions:
The test material exhibited extreme toxicty in a 48-hour, unaerated, static acute test using Daphnia magna.
Executive summary:

The rangefinding study was designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. Nominal concentrations tested: 0, 0.5, 1.0, 50, 500, 5000 mg/L. The pH and dissolved oxygen readings have been taken approximately 24 hours after exposure due to total mortality.

Results:

Based on visual observations, all solutions were clear at the test start, except for 50mg/L which was slightly cloudy, white in color. Immobilities were 0, 100, 100, 100, 100 and 100% respectively. The 48 -hour EC50, based on nominal concentrations and immobility, was less than 0.5 mg/L. All water quality parameters were within acceptable limits.

The test substance exhibited extreme toxicity in a 48 -hour, unaerated, static acute test using Daphnia magna.

Description of key information

The rangefinding study was designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. Nominal concentrations tested: 0, 0.5, 1.0, 50, 500, 5000 mg/L. The pH and dissolved oxygen readings have been taken approximately 24 hours after exposure due to total mortality.

Results:

Based on visual observations, all solutions were clear at the test start, except for 50mg/L which was slightly cloudy, white in color. Immobilities were 0, 100, 100, 100, 100 and 100% respectively. The 48 -hour EC50, based on nominal concentrations and immobility, was less than 0.5 mg/L. All water quality parameters were within acceptable limits.

The test substance exhibited extreme toxicity in a 48 -hour, unaerated, static avute test using Ceriodaphnia dubia.

The rangefinding study was designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. Nominal concentrations tested: 0, 0.5, 1.0, 50, 500, 5000 mg/L. The pH and dissolved oxygen readings have been taken approximately 24 hours after exposure due to total mortality.

Results:

Based on visual observations, all solutions were clear at the test start, except for 50mg/L which was slightly cloudy, white in color. Immobilities were 0, 100, 100, 100, 100 and 100% respectively. The 48 -hour EC50, based on nominal concentrations and immobility, was less than 0.5 mg/L. All water quality parameters were within acceptable limits.

The test substance exhibited extreme toxicity in a 48 -hour, unaerated, static acute test using Daphnia magna.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.5 mg/L

Additional information