Reaction mass of L-isoleucine and octadecan-1-ol and ethanesulphonic acid and docosan-1-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

november 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of L-isoleucine and octadecan-1-ol and ethanesulphonic acid and docosan-1-ol

EC Number:

931-105-8

Cas Number:

1156505-34-8

Molecular formula:

C26-30H56-64O5NS

IUPAC Name:

Reaction mass of L-isoleucine and octadecan-1-ol and ethanesulphonic acid and docosan-1-ol

Specific details on test material used for the study:

Test item Brassicyl Isoleucinate Esylate
Batch # D-570-069
Expiration date August 2011
Storage conditions Room temperature

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The Daphnia magna used in this toxicity test were obtained from laboratory cultures maintained at Springborn Smithers Laboratories (Europe), originally obtained from a culture at Springborn Smithers Laboratories Wareham (MA), USA.
Prior to testing, the daphnids were held in glass beakers under a photoperiod of 16 hours light and 8 hours darkness with a 30 minute transition period. The culture water consisted of modified Elendt M4 medium. After preparation the medium was aerated using an air pump to bring the pH and dissolved gases into equilibrium with the atmosphere. The modified Elendt M4 medium was allowed to equilibrate for at least two days prior to use. Total hardness, total alkalinity, pH and specific conductivity of the dilution water were determined after preparing each batch to verify that these parameters are within the acceptable ranges. Representative samples of the dilution water source used in the preparation of the culture medium were analyzed half-yearly for the presence of pesticides, PCBs and toxic metals at Bachema, Schlieren, and Interlabor Belp AG, Belp, Switzerland. None of these compounds have been detected at concentrations that are considered toxic in any of the water samples analyzed. Based on the results of these analyses and the ability of daphnids to survive and reproduce over multiple culture generations in the dilution water, it was confirmed that the dilution water used was of acceptable quality.
The batch of dilution water used in the culture three days prior to test initiation was characterized as having a total hardness and a total alkalinity as calcium carbonate (CaCO3) of 170 mg/L and of 28 mg/L, respectively, a specific conductivity of 420 μS/cm and a pH of 6.61. The temperature ranged from 19.8 to 21.0°C three days prior to test initiation.
Daphnids were fed 2.0-2.5 mL of a solution containing approximately 4 x 107 cells/mL of the unicellular green alga, Ankistrodesmus falcatus (ANK) and approximately four drops or 0.5 mL of a combination of yeast, cereal leaves and flaked fish food (YCT) daily. Representative samples of the food were analyzed periodically for the presence of pesticides, PCBs and toxic metals at Bachema, Schlieren, and Interlabor Belp AG, Belp, Switzerland. None of these compounds have been detected at concentrations that are considered toxic.

Study design

Test type:

static

Water media type:

other: Modified Elendt M4 Medium pH: 6.61 Specific conductivity: 420 μS/cm Total hardness as CaCO3: 170 mg/L Total alkalinity as CaCO3: 28 mg/L

Total exposure duration:

48 h

Test conditions

Hardness:

Total hardness as CaCO3: 170 mg/L

Test temperature:

continuously measured temperature of 20.1 to 20.5oC (single point measured temperature 20.4 to 20.9ºC)

pH:

pH: 6.61

Conductivity:

Specific conductivity: 420 μS/cm

Nominal and measured concentrations:

definitive test concentrations of 6.25, 12.5, 25.0, 50.0 and 100 mg test item/L and a control were selected for the definitive exposure.

Details on test conditions:

The test vessels were placed in a water bath in order to maintain exposure solution temperatures at 20 ± 1°C. The test vessels were 250 mL glass beakers, each containing 200 mL of test solution. The test vessels were covered with a cling film during the 48 hour exposure. The test was illuminated to a light intensity of 399 to 475 lux using fluorescent bulbs. A 16-hour light, 8-hour dark photoperiod was maintained with an automatic timer.

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

EC50 > 100 mg/L
The highest concentration tested producing no immobile daphnids was determined to be 100 mg test item /L