Registration Dossier
Registration Dossier
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EC number: 931-105-8 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of L-isoleucine and octadecan-1-ol and ethanesulphonic acid and docosan-1-ol
- EC Number:
- 931-105-8
- Cas Number:
- 1156505-34-8
- Molecular formula:
- C26-30H56-64O5NS
- IUPAC Name:
- Reaction mass of L-isoleucine and octadecan-1-ol and ethanesulphonic acid and docosan-1-ol
Constituent 1
- Specific details on test material used for the study:
- Test item Brassicyl Isoleucinate Esylate, Brassica Alcohol
Batch # D-570-069
Expiration date August 2011
Storage conditions Room temperature
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Pseudokirchneriella subcapitata
Source - Springborn Smithers Laboratories (Europe) culture
Study design
- Test type:
- static
- Water media type:
- other: Algal Growth Medium (OECD, 2006)
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- temperature of 24 ± 2ºC
- Nominal and measured concentrations:
- Definitive test concentrations of 0.30, 0.95, 3.1, 9.8, 31 and 100 mg test item/L and a dilution
water control were selected in consultation with the Study Sponsor. - Details on test conditions:
- The test was conducted in a temperature-controlled room to maintain the test conditions
specified in the study plan: a temperature of 24 ± 2ºC and continuous light intensity of 4400
to 8800 lux. An orbital shaker table provided a shaking rate of approximately 100 rpm. Test
flasks were randomly placed on the shaker table at test initiation. Following each observation
interval the test flasks were assigned new random positions.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.85 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.95 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- RESULTS 72hr EC20 > 100 mg/L / EC50 > 100 mg/L / NOEC = 0.30 mg/L based on nominal concenration and effect growth rate
- Executive summary:
STUDY PLAN TITLE: Brassicyl Isoleucinate Esylate, Brassica Alcohol: Growth inhibition test with Pseudokirchneriella subcapitata (syn. Selenastrum capricornutum) under static conditions
SPRINGBORN SMITHERS LABS. STUDY NUMBER: 1143.001.430
TEST ITEM: Brassicyl Isoleucinate Esylate, Brassica Alcohol, Lot #D-570-069, was received on 07 August 2009
TEST DATES: 28 to 31 December 2009
TEST ORGANISM: Pseudokirchneriella subcapitata / Source - Springborn Smithers Laboratories (Europe) culture
DILUTION WATER: Algal Growth Medium (OECD, 2006)
TEST CONDITIONS: 72-hour duration, continuously measured temperature of 23C, continuous illumination at 7029 to 8577 lux, shaking rate of 100 rpm
NOMINAL TEST CONCENTRATIONS: Control and 0.30, 0.95, 3.1, 9.8, 31 and 100 mg test item/L
RESULTS 72hr EC20 > 100 mg/L / EC50 > 100 mg/L / NOEC = 0.30 mg/L based on nominal concenration and effect growth rate
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