Sesbania grandiflora dry purified extract

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

Test article name : Sesbania grandiflora dry purified extract
Chemical class : : Botanical extract
Preparation date : April, 2009
Expiry date : April, 2011
Physical form at 20°C : Powder
Colour : Pale brown
Storage conditions : The product will be stored at room temperature, in well ventilated room, in its own original container
Safety precautions : Routinely hygienic procedures

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

140 - 250 mg/L as CaCO3

Test temperature:

The incubation temperature was continuously monitored during the course of the study by the means of a data logger. The measured values ranged between 20.7 and 22.3 °C

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No mortality was observed in the test medium after 24 and 48 hours, therefore the NOEC (highest concentration tested without toxic effects after the exposure period) of Sesbania grandiflora dry purified extract to Daphnia magna was determined to be equal to the nominal tested concentration of 100.0 mg/L.

Based on the observed effects, the LOEC and the 48-h IC50 were determined to be greater than the tested concentration, 100.0 mg/L.

Executive summary:

The acute toxicity of the test itemSesbania grandifloradry purified extract toDaphnia magnawas determined in a 48‑hour static test according to the EU Commission Directive 92/69/EEC, Part C.2 (1992), and the OECD Guideline for Testing of Chemicals, No. 202 (2004).

 

For this purpose, juvenile daphnids were exposed to an aqueous test medium containing the test item at the only nominal concentration of 100.0 mg/L. The exposed organisms were checked for immobilization 24 and 48 hours after test initiation.

 

The pH of the test medium after preparation was 7.49 for test item and 7.45 for control. Therefore, the pH of the test medium was not adjusted before the start of the test.

The room temperature was in the range 20.7 –during the test period, these values slightly exceeded the OECD’s recommended range (20.0±2.0 °C), however this excess did not affect the results of the study, as showed by the immobilization data in the control.

 

In the control 0% mortality was observed during the test period of 48 hours. This value complies with the validity criteria of the test according to the mentioned guidelines that provide a maximum value of mortality in the control of 10%.