Registration Dossier

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1982
Report Date:
1982
Reference Type:
other: Monograph
Title:
2,6-Dimethyl-2,4,6-Octatriene
Year:
1988
Bibliographic source:
Food and Chemical Toxicology 26 (4), 309

Materials and methods

Principles of method if other than guideline:
- Principle of test: Acute dermal toxicity in rabbits
- Short description of test conditions: 2 rabbits at high dose and 4 rabbits at other doses
- Parameters analysed / observed: LD50, mortality, systemic effects, gross necropsy
GLP compliance:
no
Test type:
other:
Limit test:
no

Test material

Reference
Name:
Unnamed
Test material form:
liquid
Specific details on test material used for the study:
The test material (E,E)-2,6-dimethylocta-2,4,6-triene (EC 221-153-1) is the constituent of the registered substance.

Test animals

Species:
rabbit
Strain:
not specified
Sex:
not specified

Administration / exposure

Type of coverage:
not specified
Vehicle:
not specified
Doses:
1250, 1770, 2140, 2500 and 5000 mg/kg bw
No. of animals per sex per dose:
2 rabbits exposed to 5000 mg/kg bw, 4 rabbits exposed to other doses
Control animals:
not specified

Results and discussion

Effect levelsopen allclose all
Sex:
not specified
Dose descriptor:
LD0
Effect level:
ca. 1 250 mg/kg bw
Based on:
test mat.
Sex:
not specified
Dose descriptor:
LD0
Effect level:
ca. 1 770 mg/kg bw
Based on:
test mat.
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
ca. 2 400 mg/kg bw
Based on:
test mat.
95% CL:
>= 2 100 - 2 600
Sex:
not specified
Dose descriptor:
LD100
Effect level:
>= 2 500 mg/kg bw
Based on:
test mat.
Sex:
not specified
Dose descriptor:
LD100
Effect level:
> 5 000 mg/kg bw
Based on:
test mat.
Sex:
not specified
Effect level:
ca. 2 140 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 1/4 animals died
Mortality:
1250 mg/kg bw: no mortality
1770 mg/kg bw: no mortality
2140 mg/kg bw: 1/4 died
2500 mg/kg bw: 4/4 died
5000 mg/kg bw: 2/2 died
Clinical signs:
Negative righting reflex, prostration, lethargy, ptosis and few feces.
Gross pathology:
Abnormalities in lungs, heart, liver and intestines.

Applicant's summary and conclusion

Conclusions:
The acute dermal LD50 of 2,6-dimethyl-2,4,6-octatriene (CAS 3016-19-1) in rabbits is 2400 mg/kg bw (95% C.I. 2100-2600 mg/kg bw). Clinical signs observed were negative righting reflex, prostration, lethargy, ptosis and few feces. Necropsy of dead animals revealed abnormalities in lungs, heart, liver and intestines.
Executive summary:

The acute dermal LD50 of 2,6-dimethyl-2,4,6-octatriene (CAS 3016-19-1) in rabbits is 2400 mg/kg bw (95% C.I. 2100-2600 mg/kg bw). Clinical signs observed were negative righting reflex, prostration, lethargy, ptosis and few feces. Necropsy of dead animals revealed abnormalities in lungs, heart, liver and intestines.