Registration Dossier

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1982
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1982
Report Date:
1982
Reference Type:
other: Monograph
Title:
2,6-Dimethyl-2,4,6-Octatriene
Year:
1988
Bibliographic source:
Food and Chemical Toxicology 26 (4), 309

Materials and methods

Principles of method if other than guideline:
- Principle of test: Acute oral toxicity in Wistar rats
- Short description of test conditions: Ten male Wistar rats were used with initial body weights of 200-287 grams. Mortality and/or systemic effects were recorded 3-4 hours after dosing and daily thereafter for 14 days. Gross necropsy was carried out on all animals. LD50 was calculated by the method of Litchield and Wilcoxon.
- Parameters analysed / observed: LD50, mortality, systemic effects, gross necropsy
GLP compliance:
no
Test type:
other: Acute oral toxicity in Wistar rats
Limit test:
no

Test material

Reference
Name:
Unnamed
Test material form:
liquid
Specific details on test material used for the study:
The test material (E,E)-2,6-dimethylocta-2,4,6-triene (EC 221-153-1) is the constituent of the registered substance.

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals and environmental conditions:
Initial body weight of rats: 200-287 g

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
unchanged (no vehicle)
Doses:
1200, 3100 and 5000 mg/kg bw
No. of animals per sex per dose:
10
Control animals:
no

Results and discussion

Effect levelsopen allclose all
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
ca. 1 900 mg/kg bw
Based on:
test mat.
95% CL:
>= 1 600 - <= 2 300
Remarks on result:
other: Calculation method: Litchield and Wilcoxon
Sex:
male
Dose descriptor:
LD0
Effect level:
ca. 1 200 mg/kg bw
Based on:
test mat.
Sex:
male
Dose descriptor:
LD100
Effect level:
>= 3 200 mg/kg bw
Based on:
test mat.
Sex:
male
Dose descriptor:
LD100
Effect level:
> 5 000 mg/kg bw
Based on:
test mat.
Mortality:
1200 mg/kg bw: 0/10 deaths
1900 mg/kg bw: 5/10 deaths
3100 mg/kg bw: 10/10 deaths
5000 mg/kg bw: 10/10 deaths.
Clinical signs:
1200 mg/kg bw: chromorhinorrhea, piloerection, lethargy, ptosis, chromodacryorrhea, ataxia and flaccid
1900 mg/kg bw: chromorhinorrhea, piloerection, lethargy, ptosis, chromodacryorrhea, ataxia and flaccid
3100 mg/kg bw: lethal - chromorhinorrhea, piloerection, lethargy, ptosis, chromodacryorrhea, ataxia and flaccid
5000 mg/kg bw: lethal - chromorhinorrhea, piloerection, lethargy, ptosis, chromodacryorrhea, ataxia and flaccid
Gross pathology:
All dead animals had abnormalities in lungs, heart, kidneys, stomach, testes and/or intestine at necropsy.

Applicant's summary and conclusion

Conclusions:
The acute oral LD50 of 2,6-dimethyl-2,4,6-octatriene (CAS 3016-19-1) in Wistar male rats is 1900 mg/kg bw (95% C.I. 1600-2300 mg/kg body weight). Clinical signs observed were chromorhinorrhea, piloerection, lethargy, ptosis, chromodacryorrhea, ataxia and flaccid. Necropsy of dead animals revealed abnormalities in lungs, heart, kidneys, stomach, testes and/or intestines.
Executive summary:

The acute oral LD50 of 2,6-dimethyl-2,4,6-octatriene (CAS 3016-19-1) in Wistar male rats is 1900 mg/kg bw (95% C.I. 1600-2300 mg/kg body weight). Clinical signs observed were chromorhinorrhea, piloerection, lethargy, ptosis, chromodacryorrhea, ataxia and flaccid. Necropsy of dead animals revealed abnormalities in lungs, heart, kidneys, stomach, testes and/or intestines.