Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-890-5
CAS number: -
analytically determined concentrations of the test substance in the
analysed test media varied in the range from 61% to 112% of the nominal
values. The mean measured values, calculated over all measurements
during the test period amounted to 79% of nominal at the concentration
of nominal 0.10 mg/L, to 81% at the concentration of nominal 0.32 mg/L,
and to 83 % at the concentration of nominal 1.0 mg/L. Therefore, the
biological results were based on the nominal concentrations as well on
the mean measured test substance concentrations.
the stock solution samples, the measured concentrations amounted to in
the mean 95% of the nominal values. The test substance was sufficiently
stable in the stock solutions under the test conditions during the
longest stock solution renewal periods of 4 days.
on observed results:
The death of one fish at the test concentrations of nominal 0.10 and
0.32 mg test substance/L on Day 4 was not determined as a significant
toxic effect of the test substance, since no clear symptoms of
intoxication were observed at the test fish at these test
concentrations, and according to the test guideline the death of one
fish is tolerated even in the control.
The reduced food uptake observed from Day 7 to 12 at the test
concentration of nominal 0.32 mg/L (some uneaten food was observed at
the bottom of the aquarium) had no significant effect on the growth rate
of the test fish and was therefore not assessed as a significant toxic
study was carried out to determine the long-term effects of the
read-across substance, amides, C12-18 (even-numbered) and C18-unsatd.,
N,N-bis(hydroxyethyl), in rainbow trout (Oncorhynchus mykiss) according
to OECD Guidelines 204 and 215, in compliance with GLP. Groups of ten
fish were exposed to the substance at nominal concentrations of 0,
0.032, 0.10, 0.32, 1.0 and 3.2 mg/L under flow through conditions for 28
d. Mortality and symptoms of toxicity were recorded daily and growth
parameters (body length and weight) were noted on Days 0 and 28.
Duplicate samples were taken from the approximate centre of the aquaria
at 0, 0.10 and 0.32 mg/L on Days 0, 7, 14, 20 and 25/28 for analytical
dose verification. At 3.2 mg/L, there was 100% mortality after one day.
At 1.0 mg/L, all fish showed clear symptoms of toxicity and 100%
mortality occurred by Day 25. No significant effect on mortality or
signs of toxicity were observed at 0.32 mg/L and lower. Further, the
mean body length and mean body wet weight of the surviving fish were not
significantly reduced compared to the control at doses of 0.32 mg/L and
lower. Growth rates at higher concentrations could not be determined due
to mortality. The analytically determined concentrations varied from
61-112% of nominal values. The mean measured values, calculated over all
measurements during the test period, amounted to 79% of nominal at 0.10
mg/L, 81% of nominal at 0.32 mg/L and 83 % of nominal at 1.0 mg/L. Under
the study conditions, the 28 d NOEC and LOEC for mortality, symptoms of
toxicity and effects on growth were established at nominal
concentrations of 0.32 and 1.0 mg/L (equivalent to mean measured
concentrations of 0.26 and 0.83 mg/L), respectively (Memmert, 1995).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again