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Physical & Chemical properties

Density

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Reference
Endpoint:
density, other
Remarks:
density
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Density data have been generated for various protein families. The data published in the cited book comes from 20 different protein families and they all have densities in the range from 1.32 g/mL to 1.42 g/mL. The density for trypsin has not been determined but since it is a protein it is expected, with very high reliability that it has density in this range.
Justification for type of information:
Density data have been generated for various protein families. The data published in the cited book comes from 20 different protein families and they all have densities in the range from 1.32 g/mL to 1.42 g/mL. The density for trypsin has not been determined but since it is a protein it is expected, with very high reliability that it has density in this range.
Reference:
Composition 0
Principles of method if other than guideline:
Determination of the sedimentation constant allows calculation of the density using the Svedberg equation. This has been performed in peer reviewed articles and summarised in Creighton "Proteins: structure and molecular properties" (1993).
GLP compliance:
no
Type of method:
other: See section 'Principles of methods if other that guidline'
Test material information:
Composition 1
Key result
Type:
density
Density:
> 1.32 - < 1.42 g/cm³
Temp.:
20 °C
Remarks on result:
other: density range for 20 different proteins from different protein-families

Creighton reviews density data retrieved from structurally and fuctionally different proteins. They all have densities in the range between 1.32 and 1.42 g/mL. We ascertain that the test enzyme also has density in this range even though not experimentally determined.

Conclusions:
Trypsin is a protein and therefore, it can be assumed that this protein has a density within the range 1.32 and 1.42 g/mL.
Executive summary:

The density has been determined for a wide range of different proteins, and this analysis showed that the density range is between 1.32 and 1.42 g/mL.

Being a protein, trypsin should also have density in this range, even though not experimentally determined.

Description of key information

The densities have been determined for a wide range of different proteins, and this analysis showed that the density range is between 1.32 to 1.42 g/mL.

Since trypsin is a protein it is assumed that it has density within this range.

Key value for chemical safety assessment

Relative density at 20C:
1.37

Additional information

We report the average value of 1.37 g/mL as a representative value of the density. It should, however, be noted that the uncertainty is expected to be in the order of +/- 0.05 g/mL.