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EC number: 232-650-8
CAS number: 9002-07-7
The purpose of this study was to screen for toxicity of endopeptidase,
batch PPF38268 towards algae. A 72h algal growth inhibition test was
performed in long cell test vials with Pseudokirchneriella subcapitata.
Algae are supplied as de-immobilized algal beads. The Algal tox kit test
follows the prescriptions of the OECD "Algal growth inhibition test"
(Guideline 201) and the ISO "Water Quality Freshwater Algal Growth
Inhibition Tests with Unicellular Green Algae" (ISO Standard 8692).
Endpoint of the study is percent inhibition (I%) of algal growth rate
compared to the control.
IC50: Calculated/estimated 50% growth inhibition compared to control
Endopeptidase, batch PPF38268 was found to inhibit growth rate at all
concentrations tested after 72h exposure, whereas no significant
inhibition was observed after 24 and 48h exposure. Inhibition of the
growth rate at 72h was found to be approximately 3% at 10 mg TOS/L, 34 %
at 30 mg TOS/L, 30% at 50 mg TOS/L, and 12 % at 100 mg TOS/L indicating
only slightly toxicity towards algal growth. There was no dose-response
relationship and because of the low observed toxicity towards algal
growth no ErC50 value could be estimated and must be > 100 mg TOS/L (>
24.7 mg aep/L). The NOEC value was determined to be < 10 mg TOS/L (< 2.5
The toxicity of trypsin in algae was not tested, however, an enzyme from
the same sub-subclass - sedolisin - was tested.
There was no dose-response relationship and because of the low observed
toxicity towards algal growth no ErC50 value could be estimated and must
be > 100 mg TOS/L (> 24.7 mg aep/L). The NOEC value was determined to be
< 10 mg TOS/L (< 2.5 mg aep/L). Due to the similarity between the two
enzymes, similar results are expected for trypsin.
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