Reaction mass of (2,4,6-trioxo-1,3,5-triazine-1,3,5(2H,4H,6H)-triyl)tri-2,1-ethanediyl triacrylate and 2-Propenoic acid, 1,1'-[[dihydro-5-(2-hydroxyethyl)-2,4,6-trioxo-1,3,5-triazine-1,3(2H,4H)-diyl]di-2,1-ethanediyl] ester

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Read-across from short-term toxicity study on one closely structural and physicol-chemical related susbtance: TMPTA

Justification for type of information:

The hypothesis for a read-across approach to data from THEICTA to target is the similarity of the chemical structures. Both the target substance (T) and the source substance (THEICTA) are acrylates. Study on another substance (TMPTA, S2) is used as supporting study. Both the target substance (T) and TMPTA (S2) are triacrylates. The main difference is the core group to which the acrylates are attached. Both core groups are shown to be non-toxic and will not contribute to the observed toxicity.
For further read-across see document attached in section 13.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across: supporting information

Reason / purpose for cross-reference:

read-across: supporting information

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

158.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

19.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Conclusions:

An acute toxicity test with Daphnia magna was conducted for THEICTA (part of the source substance). The test substance was tested for aquatic toxicity against Daphnia magna according to the OECD 202. After 48h exposure the aquatic toxicity was determined to be: EC50= 158.3 mg/l

An acute toxicity test with Daphnia magna was conducted for TMPTA (S2). The test substance was tested for aquatic toxicity against Daphnia magna according to the EU Method C.2 (Acute Toxicity for Daphnia). After 48h exposure the aquatic toxicity was determined to be: EC0 = 12.5 mg/l, EC50= 19.9 mg/l and EC100= 100 mg/l.

The hypothesis for a read-across approach to data from THEICTA to target is the similarity of the chemical structures. Both the target substance (T) and the source substance (THEICTA) are acrylates. Study on another substance (TMPTA, S2) is used as supporting study. Both the target substance (T) and TMPTA (S2) are triacrylates. The main difference is the core group to which the acrylates are attached. Both core groups are shown to be non-toxic and will not contribute to the observed toxicity.
For further read-across see document attached in section 13.

Executive summary:

An acute toxicity test with Daphnia magna was conducted for THEICTA (part of the source substance). The test substance was tested for aquatic toxicity against Daphnia magna according to the OECD 202. After 48h exposure the aquatic toxicity was determined to be: EC50= 158.3 mg/l

An acute toxicity test with Daphnia magna was conducted for TMPTA (S2). The test substance was tested for aquatic toxicity against Daphnia magna according to the EU Method C.2 (Acute Toxicity for Daphnia). After 48h exposure the aquatic toxicity was determined to be: EC0 = 12.5 mg/l, EC50= 19.9 mg/l and EC100= 100 mg/l.

The hypothesis for a read-across approach to data from THEICTA to target is the similarity of the chemical structures. Both the target substance (T) and the source substance (THEICTA) are acrylates. Study on another substance (TMPTA, S2) is used as supporting study. Both the target substance (T)  and TMPTA (S2) are triacrylates. The main difference is the core group to which the acrylates are attached. Both core groups are shown to be non-toxic and will not contribute to the observed toxicity.

For further read-across see document attached in section 13.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-06-16 to

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The test item concentrations were determined under the available analytical procedure: aliquots were removed from each prepared concentration at the commencement of the test and at 48h.

Vehicle:

no

Details on test solutions:

All testing was performed using a synthetic medium. Chemical reagents used for the preparation of this dilution water were of “analytical grade”; it is prepared according to the protocol described in standard NF EN ISO 6341.

The following stock solutions were prepared:
¿ CaCl2, 2H2O: 11.76 g per litre of water,
¿ MgSO4, 7H2O: 4.93 g per litre of water,
¿ NaHCO3: 2.59 g per litre of water,
¿ KCl: 0.23 g of per litre of water,

Dilution water was obtained by adding 25 mL of each of the stock solutions in a final volume of 1 litre and then aerating the solution until oxygen saturation.

The sum of Ca and Mg ions in dilution water was 2.5 mmol/L and ratios of Ca/Mg and Na/K were 4 and 10 respectively.

Test organisms (species):

Daphnia magna

Details on test organisms:

The test organisms used for this study was Daphnia magna Straus (Cladocera, Crustacea), clone 5, bred within the laboratory by acyclical parthenogenesis in a synthetic medium. Daphnia magna cultures were fed with a mixture of the microalgae Chlorella vulgaris and Pseudokirchneriella subcapitata. The neonates used were less than 24h old at test initiation (selected by filtration) and they were not first brood progeny.

Test animals were not fed during the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20.0 - 20.3 °C

pH:

0h: 7.9 - 8.0
48h: 7.6 - 7.7

Dissolved oxygen:

0h: 8.4 - 8.5 mg/L
48h: 7.9 - 8.1 mg/L

Details on test conditions:

Nominal concentrations:
0 (Control), 24.8, 37.5, 56.3, 84.3, 126.6, 189.8, 285.0 mg/L

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

158.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reference Item Test

The sensitivity of the test system and the methodology are evaluated monthly by performing an acute Daphnia magna toxicity test on potassium dichromate. The most recent value of 24h-EC50, obtained in June 2017, was equal to 1.08 mg/L.

The ISO 6341 norm has a validity criterion specifying that reference item EC50 values must be in the 0.6 to 2.1 mg/L range.

 

DefinitiveTest

The numbers of immobile D. magna recorded over the test period are presented in the table below:

Cumulative immobilisation (Definitive Test)

Test solution (mg/L)	Total daphnids at T0	Immobile daphnids		Mobility inhibition at 48h
		24h	48h
Control	20	0	0	0.0%
24.8	20	0	0	0.0%
37.5	20	0	0	0.0%
56.3	20	0	2	10.0%
84.3	20	0	7	35.0%
126.6	20	0	11	55.0%
189.8	20	2	12	60.0%
285.0	20	2	12	60.0%

Chemical analysis of test samples taken at 0 and 48h indicated that measured concentrations of the test item remained within 80-120% of the nominal concentrations and were maintained over the 48h test period (i.e. deviation <20%). Based on these results, exposure concentrations were based on nominal concentrations.

Environmental conditions

The appearance of the test solutions was visually checked at the beginning and at the end of the test. Solutions were found to be clear, no precipitation was observed at the end of the test.

Validation criteria

The definitive test met the validity criteria of the test guideline detailed as follows:

§ The immobilisation in the control did not exceed 10% at the end of the test (0%),

§ The dissolved oxygen concentrations remained above 3 mg/L over the test period (above 7.9 mg/L).

Validity criteria fulfilled:

yes

Conclusions:

The effects of the test item to Daphnia magna exposed for a duration of 48h with static conditions were assessed according to the OECD 202 Guideline.

Chemical analysis of test samples indicated that measured concentrations of the test item remained within 80-120% of the nominal concentrations and were maintained over the 48h test period, exposure concentrations were thus based on nominal concentrations (all results are mg/L):

EC10 = 50.0 mg/L (95% confidence level: 30.8 - 66.6 mg/L)
EC50 = 158.3 mg/L (95% confidence level: 126.1 - 215.0 mg/L)

Executive summary:

This study was designed to determine the effects of the test item toDaphnia magnaover a 48h period with static conditions, according to the OECD 202 Guideline.

 

Tests solutions were prepared from dilutions of a stock solution. Chemical analysis of test samples indicated that measured concentrations of the test item remained within 80-120% of the nominal concentrations and were maintained over the 48h test period. Exposure concentrations were thus based on nominal concentrations(all results are in mg/L): 

		95% confidence level
		Lower limit	Upper limit
EC10	50.0 mg/L	30.8	66.6
EC50	158.3 mg/L	126.1	215.0

  

The definitive test met the validity criteria of the test guideline detailed as follows:

§ The immobilisation in the control did not exceed 10% at the end of the test (0%),

§ The dissolved oxygen concentrations remained above 3 mg/L over the test period (above 7.9 mg/L).

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-01-15 - 1991-01-17

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

19.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

after 6 h     

EC0 =        100 mg/l

EC50 =       > 100 mg/l

EC100 =     > 100 mg/l

after 24h     

EC0 =        25 mg/l

EC50 =        52.9 mg/l

EC100 =     > 100 mg/l

after 48 h     

EC0 =        12.5 mg/l

EC50 =       19.9 mg/l

EC100 =      100 mg/l

Determination of the EC50 values followed the Probit method according to Finney.

For EC0 the highest tested concentration was taken at which an effect <= 10% occurred.

For EC100 the lowest tested concentration was taken at which an effect of 100% occurred.

Validity criteria fulfilled:

yes

Conclusions:

The test substance's acute toxicity to Daphnia was determined to be 19.9 mg/l.

Executive summary:

The test substance was tested for aquatic toxicity against Daphnia magna according to the EU Method C.2 (Acute Toxicity for Daphnia). After 48h exposure the aquatic toxicity was determined to be: EC0 = 12.5 mg/l, EC50= 19.9 mg/l and EC100= 100 mg/l. Based on the test substance is considered to be moderately toxic to daphnia.

Reference 4

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1988-10-25 - 1988-10-27

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Qualifier:

according to guideline

Guideline:

EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Vehicle:

yes

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

19 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

after 48 h:

EC0 = 12.5 mg/l

EC50 =18.99 mg/l

EC100 = 50 mg/l

Executive summary:

In a test following the guideline "Guideline for testing of chemicals, EG-1 of Jan. 1982, issued by the EPA, office of toxic substances" the EC50 of Daphnia magna (Straus) against Trimethylolpropantriacrylat (90% purity) was determined, using Dimethylsulfoxid (DMSO) as a solvent. After 48h the following results were obtained: EC0: = 12.5 mg/l, EC50 = 18.99 mg/l and the EC100 = 50 mg/l. Based on the test results Trimethylolpropantriacrylat was evaluated to be moderately toxic for Daphnia magna.

Description of key information

An acute toxicity test with Daphnia magna was conducted for THEICTA (part of the source substance). The test substance was tested for aquatic toxicity against Daphnia magna according to the OECD 202. After 48h exposure the aquatic toxicity was determined to be: EC50= 158.3 mg/l

An acute toxicity test with Daphnia magna was conducted for TMPTA (S2). The test substance was tested for aquatic toxicity against Daphnia magna according to the EU Method C.2 (Acute Toxicity for Daphnia). After 48h exposure the aquatic toxicity was determined to be: EC0 = 12.5 mg/l, EC50= 19.9 mg/l and EC100= 100 mg/l.

The hypothesis for a read-across approach to data from THEICTA to target is the similarity of the chemical structures. Both the target substance (T) and the source substance (THEICTA) are acrylates. Study on another substance (TMPTA, S2) is used as supporting study. Both the target substance (T)  and TMPTA (S2) are triacrylates. The main difference is the core group to which the acrylates are attached. Both core groups are shown to be non-toxic and will not contribute to the observed toxicity.

For further read-across see document attached in section 13.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

158.3 mg/L

Additional information

An acute toxicity test with Daphnia magna was conducted for THEICTA (part of the source substance). The test substance was tested for aquatic toxicity against Daphnia magna according to the OECD 202. After 48h exposure the aquatic toxicity was determined to be: EC50= 158.3 mg/l

An acute toxicity test with Daphnia magna was conducted for TMPTA (S2). The test substance was tested for aquatic toxicity against Daphnia magna according to the EU Method C.2 (Acute Toxicity for Daphnia). After 48h exposure the aquatic toxicity was determined to be: EC0 = 12.5 mg/l, EC50= 19.9 mg/l and EC100= 100 mg/l.

The hypothesis for a read-across approach to data from THEICTA to target is the similarity of the chemical structures. Both the target substance (T) and the source substance (THEICTA) are acrylates. Study on another substance (TMPTA, S2) is used as supporting study. Both the target substance (T)  and TMPTA (S2) are triacrylates. The main difference is the core group to which the acrylates are attached. Both core groups are shown to be non-toxic and will not contribute to the observed toxicity.

For further read-across see document attached in section 13.