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EC number: 915-672-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Read-across from short-term toxicity study on one closely structural and physicol-chemical related susbtance: TMPTA
- Justification for type of information:
- The hypothesis for a read-across approach to data from THEICTA to target is the similarity of the chemical structures. Both the target substance (T) and the source substance (THEICTA) are acrylates. Study on another substance (TMPTA, S2) is used as supporting study. Both the target substance (T) and TMPTA (S2) are triacrylates. The main difference is the core group to which the acrylates are attached. Both core groups are shown to be non-toxic and will not contribute to the observed toxicity.
For further read-across see document attached in section 13. - Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across: supporting information
- Reason / purpose for cross-reference:
- read-across: supporting information
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 158.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 19.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Conclusions:
- An acute toxicity test with Daphnia magna was conducted for THEICTA (part of the source substance). The test substance was tested for aquatic toxicity against Daphnia magna according to the OECD 202. After 48h exposure the aquatic toxicity was determined to be: EC50= 158.3 mg/l
An acute toxicity test with Daphnia magna was conducted for TMPTA (S2). The test substance was tested for aquatic toxicity against Daphnia magna according to the EU Method C.2 (Acute Toxicity for Daphnia). After 48h exposure the aquatic toxicity was determined to be: EC0 = 12.5 mg/l, EC50= 19.9 mg/l and EC100= 100 mg/l.
The hypothesis for a read-across approach to data from THEICTA to target is the similarity of the chemical structures. Both the target substance (T) and the source substance (THEICTA) are acrylates. Study on another substance (TMPTA, S2) is used as supporting study. Both the target substance (T) and TMPTA (S2) are triacrylates. The main difference is the core group to which the acrylates are attached. Both core groups are shown to be non-toxic and will not contribute to the observed toxicity.
For further read-across see document attached in section 13. - Executive summary:
An acute toxicity test with Daphnia magna was conducted for THEICTA (part of the source substance). The test substance was tested for aquatic toxicity against Daphnia magna according to the OECD 202. After 48h exposure the aquatic toxicity was determined to be: EC50= 158.3 mg/l
An acute toxicity test with Daphnia magna was conducted for TMPTA (S2). The test substance was tested for aquatic toxicity against Daphnia magna according to the EU Method C.2 (Acute Toxicity for Daphnia). After 48h exposure the aquatic toxicity was determined to be: EC0 = 12.5 mg/l, EC50= 19.9 mg/l and EC100= 100 mg/l.
The hypothesis for a read-across approach to data from THEICTA to target is the similarity of the chemical structures. Both the target substance (T) and the source substance (THEICTA) are acrylates. Study on another substance (TMPTA, S2) is used as supporting study. Both the target substance (T) and TMPTA (S2) are triacrylates. The main difference is the core group to which the acrylates are attached. Both core groups are shown to be non-toxic and will not contribute to the observed toxicity.
For further read-across see document attached in section 13.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-06-16 to
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The test item concentrations were determined under the available analytical procedure: aliquots were removed from each prepared concentration at the commencement of the test and at 48h.
- Vehicle:
- no
- Details on test solutions:
- All testing was performed using a synthetic medium. Chemical reagents used for the preparation of this dilution water were of “analytical grade”; it is prepared according to the protocol described in standard NF EN ISO 6341.
The following stock solutions were prepared:
¿ CaCl2, 2H2O: 11.76 g per litre of water,
¿ MgSO4, 7H2O: 4.93 g per litre of water,
¿ NaHCO3: 2.59 g per litre of water,
¿ KCl: 0.23 g of per litre of water,
Dilution water was obtained by adding 25 mL of each of the stock solutions in a final volume of 1 litre and then aerating the solution until oxygen saturation.
The sum of Ca and Mg ions in dilution water was 2.5 mmol/L and ratios of Ca/Mg and Na/K were 4 and 10 respectively. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test organisms used for this study was Daphnia magna Straus (Cladocera, Crustacea), clone 5, bred within the laboratory by acyclical parthenogenesis in a synthetic medium. Daphnia magna cultures were fed with a mixture of the microalgae Chlorella vulgaris and Pseudokirchneriella subcapitata. The neonates used were less than 24h old at test initiation (selected by filtration) and they were not first brood progeny.
Test animals were not fed during the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.0 - 20.3 °C
- pH:
- 0h: 7.9 - 8.0
48h: 7.6 - 7.7 - Dissolved oxygen:
- 0h: 8.4 - 8.5 mg/L
48h: 7.9 - 8.1 mg/L - Details on test conditions:
- Nominal concentrations:
0 (Control), 24.8, 37.5, 56.3, 84.3, 126.6, 189.8, 285.0 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 158.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effects of the test item to Daphnia magna exposed for a duration of 48h with static conditions were assessed according to the OECD 202 Guideline.
Chemical analysis of test samples indicated that measured concentrations of the test item remained within 80-120% of the nominal concentrations and were maintained over the 48h test period, exposure concentrations were thus based on nominal concentrations (all results are mg/L):
EC10 = 50.0 mg/L (95% confidence level: 30.8 - 66.6 mg/L)
EC50 = 158.3 mg/L (95% confidence level: 126.1 - 215.0 mg/L) - Executive summary:
This study was designed to determine the effects of the test item toDaphnia magnaover a 48h period with static conditions, according to the OECD 202 Guideline.
Tests solutions were prepared from dilutions of a stock solution. Chemical analysis of test samples indicated that measured concentrations of the test item remained within 80-120% of the nominal concentrations and were maintained over the 48h test period. Exposure concentrations were thus based on nominal concentrations(all results are in mg/L):
95% confidence level
Lower limit
Upper limit
EC10
50.0 mg/L
30.8
66.6
EC50
158.3 mg/L
126.1
215.0
The definitive test met the validity criteria of the test guideline detailed as follows:
§ The immobilisation in the control did not exceed 10% at the end of the test (0%),
§ The dissolved oxygen concentrations remained above 3 mg/L over the test period (above 7.9 mg/L).
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-01-15 - 1991-01-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 19.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance's acute toxicity to Daphnia was determined to be 19.9 mg/l.
- Executive summary:
The test substance was tested for aquatic toxicity against Daphnia magna according to the EU Method C.2 (Acute Toxicity for Daphnia). After 48h exposure the aquatic toxicity was determined to be: EC0 = 12.5 mg/l, EC50= 19.9 mg/l and EC100= 100 mg/l. Based on the test substance is considered to be moderately toxic to daphnia.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1988-10-25 - 1988-10-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- no
- Vehicle:
- yes
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Executive summary:
In a test following the guideline "Guideline for testing of chemicals, EG-1 of Jan. 1982, issued by the EPA, office of toxic substances" the EC50 of Daphnia magna (Straus) against Trimethylolpropantriacrylat (90% purity) was determined, using Dimethylsulfoxid (DMSO) as a solvent. After 48h the following results were obtained: EC0: = 12.5 mg/l, EC50 = 18.99 mg/l and the EC100 = 50 mg/l. Based on the test results Trimethylolpropantriacrylat was evaluated to be moderately toxic for Daphnia magna.
Referenceopen allclose all
Reference Item Test
The sensitivity of the test system and the methodology are evaluated monthly by performing an acute Daphnia magna toxicity test on potassium dichromate. The most recent value of 24h-EC50, obtained in June 2017, was equal to 1.08 mg/L.
The ISO 6341 norm has a validity criterion specifying that reference item EC50 values must be in the 0.6 to 2.1 mg/L range.
DefinitiveTest
The numbers of immobile D. magna recorded over the test period are presented in the table below:
Cumulative immobilisation (Definitive Test)
Test solution (mg/L) |
Total daphnids at T0 |
Immobile daphnids |
Mobility inhibition at 48h |
|
24h |
48h |
|||
Control |
20 |
0 |
0 |
0.0% |
24.8 |
20 |
0 |
0 |
0.0% |
37.5 |
20 |
0 |
0 |
0.0% |
56.3 |
20 |
0 |
2 |
10.0% |
84.3 |
20 |
0 |
7 |
35.0% |
126.6 |
20 |
0 |
11 |
55.0% |
189.8 |
20 |
2 |
12 |
60.0% |
285.0 |
20 |
2 |
12 |
60.0% |
Chemical analysis of test samples taken at 0 and 48h indicated that measured concentrations of the test item remained within 80-120% of the nominal concentrations and were maintained over the 48h test period (i.e. deviation <20%). Based on these results, exposure concentrations were based on nominal concentrations.
Environmental conditions
The appearance of the test solutions was visually checked at the beginning and at the end of the test. Solutions were found to be clear, no precipitation was observed at the end of the test.
Validation criteria
The definitive test met the validity criteria of the test guideline detailed as follows:
§ The immobilisation in the control did not exceed 10% at the end of the test (0%),
§ The dissolved oxygen concentrations remained above 3 mg/L over the test period (above 7.9 mg/L).
after 6 h
EC0 = 100 mg/l
EC50 = > 100 mg/l
EC100 = > 100 mg/l
after 24h
EC0 = 25 mg/l
EC50 = 52.9 mg/l
EC100 = > 100 mg/l
after 48 h
EC0 = 12.5 mg/l
EC50 = 19.9 mg/l
EC100 = 100 mg/l
Determination of the EC50 values followed the Probit method according to Finney.
For EC0 the highest tested concentration was taken at which an effect <= 10% occurred.
For EC100 the lowest tested concentration was taken at which an effect of 100% occurred.
after 48 h:
EC0 = 12.5 mg/l
EC50 =18.99 mg/l
EC100 = 50 mg/l
Description of key information
An acute toxicity test with Daphnia magna was conducted for THEICTA (part of the source substance). The test substance was tested for aquatic toxicity against Daphnia magna according to the OECD 202. After 48h exposure the aquatic toxicity was determined to be: EC50= 158.3 mg/l
An acute toxicity test with Daphnia magna was conducted for TMPTA (S2). The test substance was tested for aquatic toxicity against Daphnia magna according to the EU Method C.2 (Acute Toxicity for Daphnia). After 48h exposure the aquatic toxicity was determined to be: EC0 = 12.5 mg/l, EC50= 19.9 mg/l and EC100= 100 mg/l.
The hypothesis for a read-across approach to data from THEICTA to target is the similarity of the chemical structures. Both the target substance (T) and the source substance (THEICTA) are acrylates. Study on another substance (TMPTA, S2) is used as supporting study. Both the target substance (T) and TMPTA (S2) are triacrylates. The main difference is the core group to which the acrylates are attached. Both core groups are shown to be non-toxic and will not contribute to the observed toxicity.
For further read-across see document attached in section 13.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 158.3 mg/L
Additional information
An acute toxicity test with Daphnia magna was conducted for THEICTA (part of the source substance). The test substance was tested for aquatic toxicity against Daphnia magna according to the OECD 202. After 48h exposure the aquatic toxicity was determined to be: EC50= 158.3 mg/l
An acute toxicity test with Daphnia magna was conducted for TMPTA (S2). The test substance was tested for aquatic toxicity against Daphnia magna according to the EU Method C.2 (Acute Toxicity for Daphnia). After 48h exposure the aquatic toxicity was determined to be: EC0 = 12.5 mg/l, EC50= 19.9 mg/l and EC100= 100 mg/l.
The hypothesis for a read-across approach to data from THEICTA to target is the similarity of the chemical structures. Both the target substance (T) and the source substance (THEICTA) are acrylates. Study on another substance (TMPTA, S2) is used as supporting study. Both the target substance (T) and TMPTA (S2) are triacrylates. The main difference is the core group to which the acrylates are attached. Both core groups are shown to be non-toxic and will not contribute to the observed toxicity.
For further read-across see document attached in section 13.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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