Registration Dossier

Administrative data

Hazard for aquatic organisms

Hazard for air

Hazard for terrestrial organisms

Hazard for predators

Additional information

4-anilinobenzenediazonium hydrogen sulphate was tested for acute aquatic toxicity at two trophic levels (aquatic invertebrates and algae) according to annex VII of the REACH Regulation (EC n.1907/2006).

- EC50 48h Daphnia Magna = 0.36 mg/l (measured initial concentration; 95 % confidence limit: 0.30 – 0.44 mg/l):experimental data obtained testing the substance according to OECD TG 202.

- ErC50 72h Desmodesmus subspicatus  = 7.90 mg/l (measured initial concentrations; 95 % confidence limit: 4.19 – 15.16 mg/l): experimental data obtained testing the substance according to OECD TG 201.

Conclusion on classification

According to the CLP Regulation (EC n.1272/2008), the threshold for classification of a substance for acute aquatic toxicity is 1 mg/l. This limit refers to LC50 (96h) for fish, EC50 (48 h) for daphnia and EC50 (72 - 96 h) for algae or aquatic plants.

A study to assess the short-term toxicity to Daphnia Magna of 4-anilinobenzenediazonium hydrogen sulphate was performed and the result of the test was EC50 48h Daphnia Magna = 0.36 mg/l (measured initial concentration; 95% confidence limit: 0.30 – 0.44 mg/l)

Toxicity on algae was assessed in 72 -hour test always on 4-anilinobenzenediazonium hydrogen sulphate and EC50 for algal growth rate was 7.90 mg/l (measured initial concentrations; 95% confidence limit: 4.19 – 15.16 mg/l).

Considering the result of short-term toxicity test to Daphnia Magna (EC50 48h = 0.36 mg/l), 4-anilinobenzenediazonium hydrogen sulphate is classified H400 (acute aquatic toxicity cat.1) according to the CLP Regulation (EC n.1272/2008).

No chronic toxicity data is available from long-term studies. No further investigation is required considering that the substance is present as such in aqueous solution with naturally light exposure less than 30 minutes. Moreover, 4-anilinobenzenediazonium hydrogen sulphate is a monomer, used under strictly controlled conditions to produce the polymer.