Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates
- Molecular formula :C8H22NO12P.Mo.W
- Molecular weight : 635.02 g/mol
- Smiles notation : CN(C)c1ccccc1_O=[Mo](=O)(O{-})O{-}_O=[W](=O)(O{-})O{-}_OP(O)(O)=O
- Substance type: Organic
- Physical state : Solid

Analytical monitoring:

yes

Vehicle:

not specified

Details on test solutions:

The test substance was partially insoluble in water. Therefore, the test solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 24 hrs stirring, after stirring the stock was filtered and analytically detected the concentration found to be 0.0459 mg/L, on this concentration limit test was set up,respectively.

The test substance was stable in RO water. In house water solubility was 0.2308 mg/L in milliQ water hence stock prepared in RO water with 4g/4L by giving 24 hrs stirring, after stirring stock was analytically detected

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra Fish
- Strain: Danio rerio
- Source: Anytime Pet,Jaripatka, Nagpur
- Length at study initiation (length definition, mean, range and SD): average 1.7 cm
- Weight at study initiation (mean and range, SD): average 0.066 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod:16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

153.5 mg of CaCO3

Test temperature:

23 °C

pH:

7.1

Dissolved oxygen:

7.1 mg/l

Nominal and measured concentrations:

0.0459 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

< 0.046 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Mortality were observed

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

0.046 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 100 % mortality were observed

Sublethal observations / clinical signs:

8.1. Mortality and symptoms
Nominal	Number of		Mortality (cumulated) after
Concentration fish			3 h		6 h			24h			48h			72h				96h
(mg/l)			V1	V2	V1		V2	V1		V2	V1		V2	V1		V2		V1	V2
Control	4	4	-	-	-		-	-		-	-		-	-		-		-	-
0.0459	4	4	-	-	-		-	-		-	-		-	-		-		-	-
Visible Symptoms:							symptom (cumulated) after
Nominal	Number of
			3 h		6 h			24h			48h			72h				96h
Concentration	fish		V1	V2	V1	V2		V1	V2		V1	V2		V1	V2		V1 V2
(mg/l)
Control	4	4	-	-	-	-		-	-		-	-		-	-		-	-
0.0459	4	4	-	-	-	-		-	-		-	-		-	-		-	-

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

		Number of	pH readings after
Nominal	fish		24h		48h		72h		96h
Concentration			V1	V2	V1	V2	V1	V2	V1	V2
(mg/l)
Control	4	4	-	-	-	-	-	-	-	-
0.0459	4	4	-	-	-	-	-	-	-	-

Nominal	Number of		Oxygen content (mg/L) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-
0.0459	4	4	-	-	-	-	-	-	-	-
8.3. Test temperature
Nominal	Number of		Temperature (°C) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-
0.0459	4	4	-	-	-	-	-	-	-	-

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on Zebra Fish Danio rerio was determine to be < 0.0459 mg/l.

Executive summary:

Study was conducted to access the effect of Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The Stability of Test substance was determined as per the spectrophotometric method and found to be Stable for 4 days at 25°C. The test substance was partially insoluble in water. Therefore, the test solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 24 hrs stirring, after stirring the stock was filtered and analytically detected the concentration found to be 0.0459 mg/L, on this concentration limit test was set up,respectively. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Test consider to be valid as it fullfils all the necessary criteria which are required in the test. Firstly the mortality in the control(s) should not exceed 10 per cent (or one fish if less than ten are used) at the end of the test. Secondly the constant conditions should be maintained as far as possible throughout the test and, if necessary, semi-static or flow-through procedures should be used. The dissolved oxygen concentration must have been at least 60 per cent of the air saturation value throughout the test. Fourthly there must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80 per cent of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20 per cent, results should be based on the measured concentration. After 96 hours of exposure to test Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates to various nominal test concentrations, LC50 was determine to be < 0.0459 mg/l . Based on the LC50, it can be consider that the Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates was toxic to fishes and can be consider to be classified as aquatic chronic 1 as per the CLP classification criteria.

Description of key information

Study was conducted to access the effect of Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The Stability of Test substance was determined as per the spectrophotometric method and found to be Stable for 4 days at 25°C. The test substance was partially insoluble in water. Therefore, the test solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 24 hrs stirring, after stirring the stock was filtered and analytically detected the concentration found to be 0.0459 mg/L, on this concentration limit test was set up,respectively. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Test consider to be valid as it fullfils all the necessary criteria which are required in the test. Firstly the mortality in the control(s) should not exceed 10 per cent (or one fish if less than ten are used) at the end of the test. Secondly the constant conditions should be maintained as far as possible throughout the test and, if necessary, semi-static or flow-through procedures should be used. The dissolved oxygen concentration must have been at least 60 per cent of the air saturation value throughout the test. Fourthly there must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80 per cent of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20 per cent, results should be based on the measured concentration. After 96 hours of exposure to test Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates to various nominal test concentrations, LC50 was determine to be < 0.0459 mg/l . Based on the LC50, it can be consider that the Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates was toxic to fishes and can be consider to be classified as aquatic chronic 1 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.046 mg/L

Additional information

Study was conducted to access the effect of Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The Stability of Test substance was determined as per the spectrophotometric method and found to be Stable for 4 days at 25°C. The test substance was partially insoluble in water. Therefore, the test solution was prepared by dissolving 4 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 24 hrs stirring, after stirring the stock was filtered and analytically detected the concentration found to be 0.0459 mg/L, on this concentration limit test was set up,respectively. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Test consider to be valid as it fullfils all the necessary criteria which are required in the test. Firstly the mortality in the control(s) should not exceed 10 per cent (or one fish if less than ten are used) at the end of the test. Secondly the constant conditions should be maintained as far as possible throughout the test and, if necessary, semi-static or flow-through procedures should be used. The dissolved oxygen concentration must have been at least 60 per cent of the air saturation value throughout the test. Fourthly there must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80 per cent of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20 per cent, results should be based on the measured concentration. After 96 hours of exposure to test Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates to various nominal test concentrations, LC50 was determine to be < 0.0459 mg/l . Based on the LC50, it can be consider that the Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates was toxic to fishes and can be consider to be classified as aquatic chronic 1 as per the CLP classification criteria.