Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-nitro-, reaction products with 4-[(4-aminophenyl)azo]benzenesulfonic acid, reduced, oxidized, potassium sodium salts

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-nitro-, disodium salt, reaction products with 4-[(4-aminophenyl)azo]benzenesulfonic acid, sodium salts

EC Number:

215-397-8

EC Name:

Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-nitro-, disodium salt, reaction products with 4-[(4-aminophenyl)azo]benzenesulfonic acid, sodium salts

Cas Number:

1325-54-8

Molecular formula:

Molecular formula of the main constituents: (1) C38H24N8Na4O12S4 (2) C52H32N10Na6O18S6

IUPAC Name:

Reaction mass of Tetrasodium 5-[[4-[(4-sulfonatophenyl)diazenyl]phenyl]diazenyl]-2-[2-[2-sulfonato-4-[[4-[(4-sulfonatophenyl)diazenyl]phenyl]diazenyl]phenyl]ethenyl]benzenesulfonate and Hexasodium 2-((E)-2-sulfonato-4-((E)-(3-sulfonato-4-((E)-2-sulfonato-4-((E)-(4-((E)-(4-sulfonatophenyl)diazenyl)phenyl)diazenyl)styryl)phenyl)diazenyl)styryl)-5-((E)-(4-((E)-(4- sulfona-tophenyl)diazenyl)phenyl)diazenyl)benzenesulfonate.

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 10.0 mg of the tested substance were dissolved in 100 ml test medium to prepare a stock solution of 0.10 mg/ml. Calculated volumes of this stock solution were diluted with test medium to obtain standard solutions in the range from 0.1 mg/1 to 25.0 mg/L of tested substance.
- Sampling method: The stock solution of the biological test was diluted l000 fold with test medium. Due to precipitation effects, samples with a nomial concentration of 309 mg/1 and 1000 mg/1 sampled test must have been filtered first. Samples with a nominal concentration of 309 mg/1 were diluted l0 fold with test medium. Samples with a nomial concentration of 1000 mg/1 were diluted 50 fold with test medium.
- Sample storage conditions before analysis: The samples were stored deep-frozen (-20° C) until the analysis was performed.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
-Common name: Daphnia magna Straus (6-24 hours old)
-Source: RCC laboratories
-The parental Daphnia were maintained in the same reconstituted water as in the test at least 24 hours prior to the start of the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/l as CaCCO3

Test temperature:

19.5-19.8°C

pH:

7.9-8.7

Dissolved oxygen:

>8.2 mg/l

Salinity:

CaCl2 x 2H20 294 mg/l
MgS04 x 7H20 123 mg/l
NaHC03 65 mg/l
KCl 5.8 mg/l

Nominal and measured concentrations:

A stock solution was prepared by dissolving the test substance in test water (10.0 g/1). The stock solution was diluted in a series of sequential
dilutions with test water to prepare the following final nominal concentrations: 95, 171, 309, 556 and 1000 mg test substance/l

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: Mg= 4 : 1; Na : K = 10 : 1
- Conductivity: 0.1 microS/cm

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours/day, about 200 - 600 Lux
- Light intensity: 200-600 lux

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Up to the highest nominal concentration tested (1000 mg/L), no toxic effect on Daphnia magna was observed. Therefore, the NOEC (highest concentration tested without toxic effect) of the tested sample after 48 hours test duration is 1000 mg/L. The substance is acutely not harmful to aquatic invertebrates.

Executive summary:

The objective of the 48-hour toxicity test was to evaluate the influence of the test substance on the mobility respectively survival of Daphnia magna. The nominal concentrations tested were 95, 171, 309, 556 and 1000 mg test substance /L and a control without any additions. Up to the higest concentration tested of 1000 mg test substance/1 no toxic effect on Daphnia magna was observed. Therefore, the NOEC (highest concentration tested without toxic effect) of the test substance after 48 hours test duration is 1000 mg/1. Since according to the OECD Guideline No. 202 concentrations exceeding 1000 mg test substance/1 should not be tested, the 48 h EC 50 could not be specified.In the analysed test media the measured concentrations of the test substance only amounted to 29.2 - 41.7 % of the nominal values. These low recovery rates were probably due to a precipitation of the test substance, which was observed in test media of all test concentrations during test duration and also in the samples taken for the analytical measurements. Since in the stock solution a concentration was measured corresponding to 102.4 % of the nominal value, the reported results are related to the nominal concentrations of the test substance.