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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: screening tests
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1974
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1974
Report date:
1974

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.5 (Degradation: Biochemical Oxygen Demand)
Principles of method if other than guideline:
BSB5 : Method DEV H5
CSB: Method DEV H4
pH=9.6 (conc. 1g/l)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-nitro-, disodium salt, reaction products with 4-[(4-aminophenyl)azo]benzenesulfonic acid, sodium salts
EC Number:
215-397-8
EC Name:
Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-nitro-, disodium salt, reaction products with 4-[(4-aminophenyl)azo]benzenesulfonic acid, sodium salts
Cas Number:
1325-54-8
Molecular formula:
Molecular formula of the main constituents: (1) C38H24N8Na4O12S4 (2) C52H32N10Na6O18S6
IUPAC Name:
Reaction mass of Tetrasodium 5-[[4-[(4-sulfonatophenyl)diazenyl]phenyl]diazenyl]-2-[2-[2-sulfonato-4-[[4-[(4-sulfonatophenyl)diazenyl]phenyl]diazenyl]phenyl]ethenyl]benzenesulfonate and Hexasodium 2-((E)-2-sulfonato-4-((E)-(3-sulfonato-4-((E)-2-sulfonato-4-((E)-(4-((E)-(4-sulfonatophenyl)diazenyl)phenyl)diazenyl)styryl)phenyl)diazenyl)styryl)-5-((E)-(4-((E)-(4- sulfona-tophenyl)diazenyl)phenyl)diazenyl)benzenesulfonate.
Test material form:
solid: particulate/powder

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
5 d

Results and discussion

% Degradation
Remarks on result:
other: BOD5/COD

BOD5 / COD results

BOD5 / CODopen allclose all
Key result
Parameter:
BOD5
Value:
12 mg O2/g test mat.
Parameter:
COD
Value:
617 mg O2/g test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The substance has a BOD5 of 12 mg O2/g test mat. The ratio BOD5/COD is ca. 2, not indicating any potential for biodegradation.