Reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates

Endpoint summary

Description of key information

Short term toxicity to fish:

1. After the exposure of test chemical with fishes lethal effect at which 50% mortality were observed and measured. The LC50 was determine to be 0.6 mg/l.

2. Based on the mortality of fish by the test chemical for 96 hrs, the LC50 was determine to be 0.91 mg/l.

Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl-molybdate, tungstate & phosphates was toxic and classified as aquatic acute 1 as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

1. Based on the mobility of daphnia magna by the test chemical, the effective concentration EC50 to 50% of daphnia magna for test chemical was determine to be 0.28 mg/l at 48 hr. From EC50, It can be concluded that the chemical was toxic to the aquatic invertebrate.

2. Study was conducted for 48 hrs and after that immobility was observed. Based on the immobility the EC50 was determine to be 0.27 mg/l.

3. After the exposure of 48 hrs immobility was observed in 50 % test organisms. The EC50 value for chemical was determined to be 0.072 mg/l. Based on the value the substance concluded  to be toxic and can be considered to be classified in aquatic acute 1 as per the CLP regulations.

Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates was toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Toxicity to aquatic algae and cyanobacteria:

1. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 0.34 and 0.026 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 0.093 and 0.026 mg/l, respectively.

2. After the exposure of 72 hrs of test chemical with green algae effect on the growth rate and on area under the growth curve were observed. The EC50 and NOEC on the basis of growth rate was determine to be 0.15 and 0.027 mg/l. And on the basis of area under the growth curve the EC50 and NOEC was determine to be at 0.11 and 0.037 mg/l.

Based on above experimental studies it was concluded that the chemical reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates was toxic to the growth of algae and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Toxicity to microorganisms:

1. Based on no effect on growth inhibition of gram negative test organisms, the NOEC value of test material was determine to be 0.6918 mg/l for Erwinia herbicola and Pseudomonas syringae. Whereas the growth inhibition was observed on the other organisms so LOEC was 0.6918 mg/l for remaining 12 bacteria.

2. Based on growth inhibition of gram positive test organisms, the LOEC value of test chemical was determine to be 0.6918 mg/l.      

Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates shows low effect at 0.6194 mg/l.

Additional information

Short term toxicity to fish:

 Summarized result for data available for the structurally and functional similar read across chemicals study has been reviewed to determine the toxicity of the test reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates on the growth of fish. The studies are as mentioned below:

 

In study one short term toxicity study was carried out to determine the nature of test chemical when exposed with the fishes by providing time 96 hrs. After the exposure of test chemical with fishes lethal effect at which 50% mortality were observed and measured. The LC50 was determine to be 0.6 mg/l. Based on the LC50, chemical was consider as toxic and classified as aquatic acute 1 as per the CLP classification criteria.

 

First study was supported by second. Aim of this study was to determine the short term toxicity of test chemical on mortality of fish. Test was carried out according to the OECD Guideline 203 (Fish, Acute Toxicity Test) 2-benzylideneheptanal under the static system. Based on the mortality of fish by the test chemical for 96 hrs, the lethal concentration LC50 was determine to be 0.91 mg/l. It can be concluded from the LC50 value that the test chemical was toxic to the fish and thus can be considered to be classified in "aquatic acute category 1" as per the CLP classification criteria.

 

Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates was toxic and classified as aquatic acute 1 as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

Summarized result for data available for the structurally and functional similar read across chemicals study has been reviewed to determine the toxicity of the test chemical Reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates on the growth of aquatic invertebrates. The studies are as mentioned below:

 

In study 1 short term toxicity test was carried out for test chemical according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) on Daphnia magna for 48 hr exposure period. Test conducted under the static system and provide proper atmosphere as mention in the guideline. Based on the mobility of daphnia magna by the test chemical, the effective concentration EC50 to 50% of daphnia magna for test chemical was determine to be 0.28 mg/l at 48 hr. From EC50, It can be concluded that the chemical was toxic to the aquatic invertebrate and thus can be considered to be classified in aquatic acute category 1 as per the classification criteria.    

 

First study was supported by second in which aim of this study was to evaluate the nature of chemical when comes in contact with organisms by providing the exposure period of 48 hrs. Test conducted under the static system. Study was conducted for 48 hrs and after that immobility was observed. Based on the immobility the EC50 was determine to be 0.27 mg/l. Based on the immobility, chemical was concluded as toxic and classified as aquatic acute 1/ chronic 2 as per the CLP criteria.

 

Similarly in third study short term toxicity to aquatic invertebrates was performed in static salt water condition for 48 hrs. <24 hrs old daphnia were used as a test organism. Test conducted under the static system in which 5 daphnia added in each vessel. After the exposure of 48 hrs immobility was observed in 50 % test organisms. The EC50 value for chemical was determined to be 0.072 mg/l. Based on the value the substance concluded to be toxic and can be considered to be classified in aquatic acute 1 as per the CLP regulations.

 

Based on above experimental studies it was concluded that the chemical reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates was toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

 

 

Toxicity to aquatic algae and cyanobacteria:

 

Summarized results of the data available for the structurally and functional similar read across chemicals study has been reviewed to determine the toxicity of the test reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates on the growth of aquatic algae. The studies are as mentioned below:

 

In the first study short term toxicity to green algae study was carried out for 72 hrs. The study was based on the effects of the test compound on green algae in a static fresh water system. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 0.34 and 0.026 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 0.093 and 0.026 mg/l, respectively. Thus, based on the EC50 value, it can be concluded that the substance can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic acute category 1 / chronic 1 as per the CLP classification criteria.

 

Similarly aim of the second study was to evaluate the nature of chemical when comes in contact with green algae by providing the exposure period of 72 hrs. Test conducted under the static system. After the exposure of 72 hrs of test chemical with green algae effect on the growth rate and on area under the growth curve were observed. The EC50 and NOEC on the basis of growth rate was determine to be 0.15 and 0.027 mg/l. And on the basis of area under the growth curve the EC50 and NOEC was determine to be at 0.11 and 0.037 mg/l.

 

Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates was toxic to the growth of algae and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

 

 

Toxicity to microorganisms:

Summarized results of the data available for the structurally and functional similar read across chemicals study has been reviewed to determine the toxicity of the test reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates on the growth of microorganisms. The studies are as mentioned below:

 

Determination of toxicity of test material on the growth of 14 gram negative microorganisms. Inhibitory activity of test chemical was determined by the traditional agar gel method. The conc. of test chemical used for the study was 0.6918 mg/l (1 µM). 14 different gram negative bact. were used for the study was Agrobacterium radiobacter, Agrobacterium tumefaciens, Bradyrhizobium japonicum, Escherichia coli, Erwinia atroseptica,Erwinia herbicola, Erwinia uredovora, Pseudomonas fluorescence, Pseudomonas phaseolicola, Pseudomonas syringae , Rhizobium trifolii, Xanthomonas malvacearum, Xanthomonas phaseoli and X. stewartii. These test organisms were taken from the collection of the Plant Protection Institute, Hungarian Academy of Sciences (Budapest, Hungary). The bacteria were maintained on Nutrient Agar (Oxoid CM3) completed with vitamins pyridoxine. HCl, thiamine. HCl, riboflavine and nicotinamide at 1, 10, 1 and 20 mg/l concentrations. Bacterial suspensions for screening were prepared by washing cells with sterile tap water containing 0.3% peptone from slants of 20 h old cultures grown at (21±1) °C. A layer of 5 mm depth of inoculated medium was dispensed into Petri dishes of 90 mm diameter. Filter paper discs of 5 mm diameter were impregnated with 1 µM solution of test compounds and placed centrally on the surface of the agar plate. Growth inhibition zones were measured after 24 h incubation at 21 ± 1 °C. After incubation colony diameters were determined and growth inhibition was calculated in percentage of dye free colony growth. Each determination was run in quadruplicate. Based on no effect on growth inhibition of gram negative test organisms, the NOEC value of test material was determine to be 0.6918 mg/l for Erwinia herbicola and Pseudomonas syringae. Whereas the growth inhibition was observed on the other organisms so LOEC was 0.6918 mg/l for remaining 12 bacteria.

 

 

Determination of toxicity of test chemical on the growth of 8 gram positive microorganisms. Inhibitory activity of test chemical was determined by the traditional agar gel method. The conc. of test chemical used for the study was 0.6918 mg/l (1 µM).

8 different gram positive bact. were used for the study-

Gram – positive bacteria:

Corynebacterium fascians

Corynebacterium flaccumfaciens

Corynebacterium michiganense

Corynebacterium oortii

Stapylococcus aureus

Micrococcus luteus

Bacillus subtilis and

Bacillus thuringiensis Berliner subp. kurstaki

These test organisms were taken from the collection of the Plant Protection Institute, Hungarian Academy of Sciences (Budapest, Hungary). The bacteria were maintained on Nutrient Agar (Oxoid CM3) completed with vitamins pyridoxine. HCl, thiamine. HCl, riboflavine and nicotinamide at 1, 10, 1 and 20 mg/l concentrations. Bacterial suspensions for screening were prepared by washing cells with sterile tap water containing 0.3% peptone from slants of 20 h old cultures grown at (21±1) °C. A layer of 5 mm depth of inoculated medium was dispensed into Petri dishes of 90 mm diameter. Filter paper discs of 5 mm diameter were impregnated with 1 µM solution of test compounds and placed centrally on the surface of the agar plate. Growth inhibition zones were measured after 24 h incubation at 21 ± 1 °C. After incubation colony diameters were determined and growth inhibition was calculated in percentage of dye free colony growth. Each determination was run in quadruplicate. Based on growth inhibition of gram positive test organisms, the LOEC value of test substance was determine to be 0.6918 mg/l.

 

 

Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates shows low effect at 0.6194 mg/l.