Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Acute and Chronic Effects of Gallium Chloride (GaCl3) on Tilapia (Oreochromis mossambicus) Larvae
Author:
H.C. Lin and P.P. Hwang
Year:
1998
Bibliographic source:
Bulletin of Environmental Contamination and Toxicology. 60: 931-935

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
96-h acute toxicity test with Tilapia (Oreochromis mossambicus) larvae.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
not specified

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other: Oreochromis mossambicus
Details on test organisms:
Tilapia larvae are available year-round from mature adults if conditions are controlled at 26-29°C under a photoperiod of 12-14 h lighting. Mature adult tilapia obtained from the Tainan Branch of the Taiwan Fisheries Research Institute were reared in circulating freshwater under the conditions described above. Fertilized eggs and hatched larvae were incubated under the same condition as above.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
No data
Test temperature:
26-29°C
pH:
no data
Dissolved oxygen:
no data
Salinity:
no data
Conductivity:
no data
Nominal and measured concentrations:
no data
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
36 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 3-day old larvae
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
36 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 3-day old larvae
Duration:
2 d
Dose descriptor:
NOEC
Effect conc.:
102.2 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
morphology
Remarks:
body length
Remarks on result:
other: 3-day old larvae
Duration:
2 d
Dose descriptor:
LOEC
Effect conc.:
204.5 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
morphology
Remarks:
body length
Remarks on result:
other: 3-day old larvae
Duration:
2 d
Dose descriptor:
NOEC
Effect conc.:
20.4 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
morphology
Remarks:
yolk length
Remarks on result:
other: 3-day old larvae
Duration:
2 d
Dose descriptor:
LOEC
Effect conc.:
102.2 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
morphology
Remarks:
yolk length
Remarks on result:
other: 3-day old larvae
Duration:
2 d
Dose descriptor:
NOEC
Effect conc.:
4.1 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: body Na, body K, body Ca and body Mg
Remarks on result:
other: 3-day old larvae
Duration:
2 d
Dose descriptor:
LOEC
Effect conc.:
20.4 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: body Na, body K, body Ca and body Mg
Remarks on result:
other: 3-day old larvae
Duration:
2 d
Dose descriptor:
NOEC
Effect conc.:
>= 204.5 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
morphology
Remarks:
body length
Remarks on result:
other: 30-day old larvae
Duration:
2 d
Dose descriptor:
LOEC
Effect conc.:
> 204.5 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
morphology
Remarks:
body length
Remarks on result:
other: 30-day old larvae
Duration:
2 d
Dose descriptor:
NOEC
Effect conc.:
>= 204.5 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: body Na, body K, body Ca and body Mg
Remarks on result:
other: 30-day old larvae
Duration:
2 d
Dose descriptor:
LOEC
Effect conc.:
> 204.5 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: body Na, body K, body Ca and body Mg
Remarks on result:
other: 30-day old larvae

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
The 96-h LC50 for effect of GaCl3 on Tilapia (Oreochromis mossambicus) larvae is 36 mg/L.