3-hydroxypropiononitrile

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Range-finding study.

Data source

Reference

Reference Type:

publication

Title:

The place of the range finding test in the industrial toxicology laboratory.

Author:

Smyth HF, Carpenter CP

Year:

1944

Bibliographic source:

Journal of Industrial Hygiene and Toxicology 26: 269-273

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Range-finding Test for the determination of the approximate lethal dose with about six animals, refering to acute toxicity via inhalation -
method is described in more detail in the field "information on materials and methods"

GLP compliance:

not specified

Test type:

other: Range Finding test

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxypropiononitrile

EC Number:

203-704-8

EC Name:

3-hydroxypropiononitrile

Cas Number:

109-78-4

Molecular formula:

C3H5NO

IUPAC Name:

3-hydroxypropanenitrile

Constituent 2

Reference substance name:

Ethylene cyanohydrin

IUPAC Name:

Ethylene cyanohydrin

Details on test material:

- Name of test material (as cited in the study report): Ethylene cyanohydrin
- Substance type: aliphatic nitrile
- Physical state: liquid
- Analytical purity: no data
- Impurities (identity and concentrations): no data

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Wista strain, albino
- Age at study initiation: no data
- Weight at study initiation: 90-120 g
- Fasting period before study: no data
- Housing: no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS:
no data

IN-LIFE DATES: From/To: no data

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus/- Source and rate of air:
Exposure to flowing stream of air, saturated with vapor of Ethylenen cyanohydrin, prepared by passing it through a fritted disc bubbler
at room temperature

- Exposure chamber volume: no data
- Method of holding animals in test chamber: no data

- Method of conditioning air: no data
- Treatment of exhaust air: no data
- Temperature, humidity, pressure in air chamber: no data

TEST ATMOSPHERE
- Brief description of analytical method used: no data
- Samples taken from breathing zone: no data

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

>= 2 - <= 8 h

Remarks on duration:

Saturated vapour exposures for periods of time, falling in a geometric series with a constant ratio of two, up to eight hours as a maximum.

Concentrations:

saturated atmosphere

No. of animals per sex per dose:

6

Control animals:

not specified

Details on study design:

Method:
Groups of six male albino rats are exposed to a flowing stream of air substantially saturated with vapours  of the test material, prepared by passing it through a fritted disc bubbler at room temperature for periods of time falling  in a geometric series with a constant ratio of two, up to eight hours as a maximum. This practice is repeated on new groups of rats until an exposure time is found, which kills all of six, and another time which kills  none of six, within two weeks after inhalation. By this type of test the hazard rather than the toxicity of the test substance ethylene cyanohydrin is measured, 
thus the test combines the toxic effect of the vapor (via inhalation) to the test organism in dependance of the vapor pressure (quantity of the test 
substance)  and  time of exposure (inhalation time). In this case, the results of the Range finding test (refering to inhalation) for the test substance
 Ehylene  cyanohydrin, have been compared by Smyth et al. with "Carbitol" acetat (Diethylene glycol ethyl ether acetat), e.g. "Carbitol" acetat is taken 
as the "designate compound having the respective hazard in the same order of magnitude as material studied". 

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

other: inhalation

Exp. duration:

8 h

Remarks on result:

other: Animals exposed to saturated air with Ethylene cyanohydrin; at room temperature resulted in no mortality.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

Since the animals, which were exposed to saturated air for 8 hours with Ethylene cyanohydrin, showed no mortality and no other pathologic signs,
the test substance can be classified as pratically nontoxic.