O-isobutyl ethylthiocarbamate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: published data

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

2-methylpropan-1-ol (Isobutyl alcohol ) is both reagents used in the manufacture of O-isobutyl ethylthiocarbamate . Therefore, the Biodegradation in water of Isobutyl alcohol need to be considered in the assessment of O-isobutyl ethylthiocarbamate

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

Principles of method if other than guideline:

Coarse-filtered mixture of domestic treatment plant effluents and rich soil microorganisms were added to BOD dilution water at a concentration of 0.1
mL per liter. BOD dilution water is fortified with specified minerals and buffered to pH 7.2. Seven BOD bottles were prepared with and without test
substance added. One was measured for DO immediately and duplicate bottles measured at days 5, 15, and 28 using a YSI dissolved O2 meter.
Bottles were incubated at 20° C. DO measurements for the test and standard substance (ethylene glycol) were corrected for the blank values.

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

other: seed (settled sewage-treated effluent, or commercial Polyseed BOD seed)

Details on inoculum:

Coarse-filtered mixture of domestic treatment plant effluents and rich soil microorganisms were added to BOD dilution water at a concentration of 0.1 mL per liter.

Duration of test (contact time):

28 d

Initial conc.:

2 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

- Sealed 300 ml bottle
- Temperature 20°C
- BOD dilution water is fortified with specified minerals and buffered to pH 7.2.
- Seven BOD bottles were prepared with and without test substance added.
- One was measured for DO immediately and duplicate bottles measured at days 5, 15, and 28 using a YSI dissolved 02 meter.
- Bottles were incubated at 20 °C.
- DO measurements for the test and standard substance (ethylene glycol) were corrected for the blank values.

Reference substance:

not specified

Parameter:

% degradation (O2 consumption)

Value:

> 70 - < 80

Sampling time:

28 d

Details on results:

Kinetic of test substance (in %):
= 14 after 5 day(s)
= 74 after 28 day(s)

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

2-methylpropan-1-ol (Isobutyl alcohol ) is both reagents used in the manufacture of O-isobutyl ethylthiocarbamate . The ready biodegradability of the substance was examined. After 28 days, approximately 74% of the test material was degraded.
Therefore, the Biodegradation in water of Isobutyl alcohol need to be considered in the assessment of O-isobutyl ethylthiocarbamate
These results suggest that O-isobutyl ethylthiocarbamate will be readily biodegradable and it is not expected to persist in the environment.

Executive summary:

2-methylpropan-1-ol (Isobutyl alcohol ) is both reagents used in the manufacture of O-isobutyl ethylthiocarbamate .The ready biodegradability of the substance was examined. After 28 days, approximately 74% of the test material was degraded.

Therefore, the Biodegradation in water of Isobutyl alcohol need to be considered in the assessment of O-isobutyl ethylthiocarbamate

These results suggest that O-isobutyl ethylthiocarbamate will be readily biodegradable and it is not expected to persist in the environment.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: published data

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

2-methylpropan-1-ol (Isobutyl alcohol ) is both reagents used in the manufacture of O-isobutyl ethylthiocarbamate . Therefore, the Biodegradation in water of Isobutyl alcohol need to be considered in the assessment of O-isobutyl ethylthiocarbamate

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Principles of method if other than guideline:

Wastewater from a domestic treatment plant (Marl-West, Germany) were added to BOD dilution water at a concentration of 0.5 mL per liter. BOD dilution water is fortified with specified minerals and buffered to pH 7.2. Replicate BOD bottles were prepared with and without test substance added or with Texapon as a positive control. DO was measured with an O2 meter on days 5, 15, and 30. Bottles were incubated at 20 deg. C. DO measurements in the bottles without any TS showed O2 consumption of 0.9 mg/L (below the maximum desired O2 consumption for blanks of 1.5 mg/L). Percent degradation was calculated as percent theoretical oxygen demand (2.59 mg O2/mg TS).

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

other: Sewage sludge from a municipal sewage treatment in Marl, Germany, nonacclimated

Details on inoculum:

Wastewater from a domestic treatment plant (Marl-West, Germany) were added to BOD dilution water at a concentration of 0.5 mL per liter. BOD dilution water is fortified with specified minerals and buffered to pH 7.2. Replicate BOD bottles were prepared with and without test substance added or with Texapon as a positive control. DO was measured with an O2 meter on days 5, 15, and 30. Bottles were incubated at 20 deg. C. DO measurements in the bottles without any TS showed O2 consumption of 0.9 mg/L (below the maximum desired O2 consumption for blanks of 1.5 mg/L). Percent degradation was calculated as percent theoretical oxygen demand (2.59 mg O2/mg TS).

Duration of test (contact time):

30 d

Initial conc.:

2 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Wastewater from a domestic treatment plant (Marl-West, Germany) were added to BOD dilution water at a concentration of 0.5 mL per liter. BOD dilution water is fortified with specified minerals and buffered to pH 7.2. Replicate BOD bottles were prepared with and without test substance added or with Texapon as a positive control. DO was measured with an O2 meter on days 5, 15, and 30. Bottles were incubated at 20 deg. C. DO measurements in the bottles without any TS showed O2 consumption of 0.9 mg/L (below the maximum desired O2 consumption for blanks of 1.5 mg/L). Percent degradation was calculated as percent theoretical oxygen demand (2.59 mg O2/mg TS).

Reference substance:

not specified

Parameter:

% degradation (O2 consumption)

Value:

75

Sampling time:

30 d

Details on results:

Kinetic of test Subst:
5 day = 55%,
15 day = 73%,
30 days = 75%

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

2-methylpropan-1-ol (Isobutyl alcohol ) is both reagents used in the manufacture of O-isobutyl ethylthiocarbamate . Therefore, the Biodegradation in water of Isobutyl alcohol need to be considered in the assessment of O-isobutyl ethylthiocarbamate
These results suggest that O-isobutyl ethylthiocarbamate will be readily biodegradable and it is not expected to persist in the environment.

Executive summary:

2-methylpropan-1-ol (Isobutyl alcohol ) is both reagents used in the manufacture of O-isobutyl ethylthiocarbamate . Therefore, the Biodegradation in water of Isobutyl alcohol need to be considered in the assessment of O-isobutyl ethylthiocarbamate

These results suggest that O-isobutyl ethylthiocarbamate will be readily biodegradable and it is not expected to persist in the environment.

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Data waiving:

other justification

Justification for data waiving:

other:

Description of key information

According to “ANNEX IX- STANDARD INFORMATION REQUIREMENTS FOR SUBSTANCES MANUFACTURED OR IMPORTED IN QUANTITIES OF 100 TONNES OR MORE”, a simulation testing on ultimate degradation in surface water, the study does not need to be performed if the substance is ready biodegradable. Isobutyl alcohol is reagents used in the manufacture of O-isobutyl ethylthiocarbamate.Therefore, the Biodegradation in water of Isobutyl alcohol need to be considered in the assessment of O-isobutyl ethylthiocarbamate .  The ready biodegradability of the substance was examined. After 28 days, approximately 74% of the test material was degraded.

The results suggest that Isobutyl alcohol is readily biodegradable and therefore O-isobutyl ethylthiocarbamate will be readily biodegradable and it is not expected to persist in the environment. As O-isobutyl ethylthiocarbamate is ready biodegradable a ready biodegradability study does not need to be conducted.

Therefore testing for Biodegradation in water screening tests does not need to be performed.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable