Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate

EC Number:

304-762-8

EC Name:

Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate

Cas Number:

94279-16-0

Molecular formula:

C40H24N10Na4O19S4

IUPAC Name:

tetrasodium 4-[(1E)-2-{2,6-dihydroxy-3,5-bis[(1E)-2-{4-[(4-nitro-2-sulfonatophenyl)amino]phenyl}diazen-1-yl]phenyl}diazen-1-yl]-5-hydroxynaphthalene-2,7-disulfonate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Remarks:

water

Details on test solutions:

The mother solution was prepared dissolving 1,5385 g of active substance in 1000 ml of water for a final concentration of 1008,2 mg/l
The stock solutions were prepared from the mother solution.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Supplier: P & S, S.L
Lenght (cm): 2.86 ± 0,18)
Weight (g): 0.29 ± 0,12

Temperature: 22 °C ± 2
Photoperiod: 12 h light/ 12 h dark
Food: commercial feed fpr fish
Frequency: 3 times/week
Acclimatation period: minim 12 days
Mortality in acclimation period: 0%

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

120 mg CaCO3/L

Test temperature:

22 °C ± 2

pH:

8,11

Dissolved oxygen:

> 70%

Nominal and measured concentrations:

0,10 mg/L, 1,01 mg/L, 10,01 mg/L and 100,8 mg/L of active indredient

Details on test conditions:

Fish tank volume 3L
1 tank for concentration
7 fish for tank

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The results show no toxicity on fish.

Executive summary:

The test performed on the tested item shows no toxicity on the test organisms with a LC50 > 100 mg/l.