Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

no

Remarks:

not GLP study

Test material

Test material information

Constituent 1

Reference substance name:

Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate

EC Number:

304-762-8

EC Name:

Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate

Cas Number:

94279-16-0

Molecular formula:

C40H24N10Na4O19S4

IUPAC Name:

tetrasodium 4-[(1E)-2-{2,6-dihydroxy-3,5-bis[(1E)-2-{4-[(4-nitro-2-sulfonatophenyl)amino]phenyl}diazen-1-yl]phenyl}diazen-1-yl]-5-hydroxynaphthalene-2,7-disulfonate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Source (laboratory, culture collection): Cutlure Collection Algae Protozoo
- Method of cultivation:
temperature: 22+/-2°C,
Light intesity2500Lux
agitation: continuos

Study design

Test type:

static

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22+/-2°C

Nominal and measured concentrations:

1, 2.2, 4.8, 10, 22, 48 and 100 mg/l active material

Details on test conditions:

TEST SYSTEM
- Test vessel:glass tubes-40ml
- Type (delete if not applicable): closed
- Renewal rate of test solution (frequency/flow rate): every 48h
- No. of vessels per concentration (replicates):2
- No. of vessels per control (replicates):2


OTHER TEST CONDITIONS
- Light intensity and quality: 2500 Lux

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

IC50

Effect conc.:

ca. 12.8 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Results with reference substance (positive control):

IC50 72h = 1.1 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance was tested for acute toxicity to algae following OECD 201. Under the experimental conditions the IC50,72h is equal to 12.8 mg/l.

Executive summary:

The substance was tested for acute toxicity to algae following OECD 201. Under the experimental conditions the IC50,72h is equal to 12.8 mg/l.

The substance showed toxic effects to daphnia and needs to be classified under Regulation 1272/2008 (CLP) as Aq. Chronic 3, H412.