Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Physical & Chemical properties

Vapour pressure

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

The vapor pressure of four different enzymes (freeze-dried) was measured using the vapor pressure balance method at temperatures 40-50 °C and 60-70 °C (below denaturing temperature). Several runs were conducted for each enzyme and a mean value was caculated for each enzyme. All enzymes had vapor pressure in the order of 10-3 Pa.

The value presented below is an average of the vapor pressure of the 4 tested enzymes and it is assumed to be similar for enzymes in general.

Key value for chemical safety assessment

Vapour pressure:
0.004 Pa
at the temperature of:
25 °C

Additional information

The vapor pressure of enzymes is assumed to be extremely low (1-4). This is supported by the conclusion drawn for several enzymes (proteases, amylases, cellulases, lipases) mentioned in HERA reports (1, 2). These reports also concluded that the vapor pressure of these enzymes is below the lowest vapor pressure that can be analytically determined, which was 10-6Pa at the time these reports were issued. Also, NJDOH (3) and NIOSH (4) have mentioned a vapor pressure of approximately 0 mmHg vapor pressure for subtilisin.


1) HERA (2005) Human & Environmental Risk Assessment (HERA) on ingredients of household cleaning products α-Amylases, Cellulases And Lipases, 1st edition.

2) HERA (2007) Human & Environmental Risk Assessment (HERA) on ingredients of household cleaning products - Subtilisins (Protease), 2ndedition.

3) NJDOH (2003) New Jersey Department of Health and Senior Services – Hazardous Substances Fact Sheet: Subtilisins, available via: (accessed 13/08/2019).

4) NIOSH (2018) The National Institute for Occupational Safety and Health (NIOSH) – Subtilisins, available via: (accessed 13/08/2019).