Reaction products of Glycerin formal and 2-Propenoic acid, 2-methyl-, methyl ester

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30.03.2015 to 01.06.2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of Glycerin formal and 2-Propenoic acid, 2-methyl-, methyl ester

EC Number:

945-527-5

Cas Number:

1620329-57-8

Molecular formula:

C8H12O4

IUPAC Name:

Reaction products of Glycerin formal and 2-Propenoic acid, 2-methyl-, methyl ester

Test material form:

liquid

Specific details on test material used for the study:

I

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Non-adapted activated sludge from the aeration tank of a municipal biological waste water treatment plant.
30 mg/l dry matter in the final mixture.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

60.5 mg/L

Based on:

test mat.

Remarks:

Test suspension

Initial test substance concentration 2

Initial conc.:

101.2 mg/L

Based on:

ThOD

Remarks:

Test suspension

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Activated sludge was used after sampling from the treatment plant without adaptation. However, the sludge was pre-conditioned for 3 days (aerated but not
fed) to reduce the amount of O2 consumed by the blank controls. Prior to the test the sludge was washed twice with tap water and once with mineral medium. After
centrifugation the sludge, at a concentration twice of the final concentration to be achieved for the test, was suspended in test medium.The test item was applied by direct addition in the test vessels, so as to give a final
test concentration as ThOD of about 100 mg O2/l.
The reference item was dissolved in the test medium at a concentration twice of the final concentration to be achieved for the test. An appropriate amount of this stock
solution was diluted with the sludge suspension to give a final test concentration as ThOD of about 100 mg O2/l.
In the toxicity control the test item and the reference item were tested as a mixture, so as to give a final test concentration as ThOD of about 200 mg/l ; application by
direct addition and stock solution, respectively.
In the abiotic sterile control the test item was applied by direct addition in the test vessels, so as to give a final test concentration as ThOD of about 100 mg O2/l.
For each test series the following test flasks were set up:
T: Test suspension containing inoculum, test medium and test item (two
replicates)
B: Blank control containing inoculum and test medium (two replicates)
R: Procedure control containing inoculum, test medium and sodium benzoate as
ready biodegradable reference item (two replicates)
X: Toxicity control containing inoculum, test medium, test item and sodium
benzoate as ready biodegradable reference item
C: Abiotic sterile control containing test item, deionised water and 0.2 mM HgCl2
as sterilizing agent to prevent microbial decomposition
The test vessels were stirred by an inductive stirring system for a maximum test
period of 28 days. During the test the O2 uptake was continuously measured with a
manometric BOD measuring device. The pH-value was checked at the beginning
and at the end of the test. The value at the beginning was adjusted to pH 7.2 (± 0.2)
with NaOH or HCl if necessary.
The biodegradation of the test item was followed by daily measurements. Additionally, ultimate biodegradation was determined based on the theoretical
carbon content and the analysis of the dissolved organic carbon (DOC) concentrations at the end of the test.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

40

Sampling time:

28 d

Remarks on result:

other: 32 % at the end of the 10-day window

Details on results:

Determination of biodegradation
Biodegradation of Glycerolformal methacrylate based on O2 consumption was calculated to be 40% after 28 days, as compared to the theoretical oxygen demand (ThOD).
The biodegradation of Glycerolformal methacrylate reached 32% at the end of the 10-d window.
Biodegradation of the test item was observed after a lag phase of about 1 day.
The procedure control sodium benzoate reached 77% biodegradation after 14 days, thus confirming the suitability of the inoculum and of the test conditions.
The respective concentrations of organic carbon at the beginning (as mg TOC/l) and at the end (as mg DOC/l) of the test after 28 days were, respectively:
33.81) and 21.8 for the test units (mean of two replicates) <0.5 and 1.00 for the blank control (mean of two replicates)
35.01) and 1.08 for the procedure control (mean of two replicates) 1) theoretical value calculated based on the molecular formula The calculated biodegradation based on DOC measurement reached 37% for
Glycerolformal methacrylate and 100% for sodium benzoate. The data essentially confirm the limited biodegradability of the test item.
Glycerolformal methacrylate did not reach the pass level of 60% for ready biodegradability in the Manometric Respirometry Test, neither within the 10-d window nor after 28 days of incubation and, therefore, cannot be termed as readily biodegradable.

Any other information on results incl. tables

O2 uptake of the test units, the blank control and the corresponding degradation data

 

Time (days)	Blankcontrol	Testunit no.1 containingtestitem		Testunit no.2containingtestitem		MeanDegradationofno.1+2(%)
	BOD (mgO2/l)*	BOD (mgO2/l)	Degradation(%)**	BOD (mgO2/l)	Degradation(%)**
0	0.0	0.0	0.0	0.0	0.0	0.0
1	4.1	4.1	0.0	4.1	0.0	0.0
2	9.3	10.3	1.0	10.3	1.0	1.0
3	12.4	16.5	4.1	14.4	2.0	3.0
4	12.4	20.6	8.1	20.6	8.1	8.1
5	15.5	28.9	13.3	33.0	17.3	15.3
6	16.5	37.1	20.4	41.3	24.5	22.4
7	18.6	41.3	22.4	43.3	24.4	23.4
8	18.6	45.4	26.5	47.5	28.6	27.5
9	20.6	49.5	28.6	49.5	28.6	28.6
10	20.6	49.5	28.6	49.5	28.6	28.6
11	20.6	51.6	30.6	51.6	30.6	30.6
12	20.6	53.6	32.6	51.6	30.6	31.6
13	21.7	55.7	33.6	53.6	31.6	32.6
14	22.7	55.7	32.6	55.7	32.6	32.6
15	23.8	59.8	35.6	59.8	35.6	35.6
16	23.8	61.9	37.7	61.9	37.7	37.7
17	24.8	61.9	36.7	64.0	38.7	37.7
18	24.8	64.0	38.7	64.0	38.7	38.7
19	23.8	64.0	39.8	64.0	39.8	39.8
20	25.8	64.0	37.7	66.0	39.7	38.7
21	24.8	64.0	38.7	66.0	40.7	39.7
22	23.8	64.0	39.8	66.0	41.7	40.8
23	26.8	66.0	38.7	68.1	40.8	39.8
24	25.8	68.1	41.8	68.1	41.8	41.8
25	26.8	66.0	38.7	68.1	40.8	39.8
26	26.8	68.1	40.8	68.1	40.8	40.8
27	27.9	70.2	41.8	70.2	41.8	41.8
28	28.9	68.1	38.7	70.2	40.8	39.8

 

* Mean of two replicates

** The calculation is based on a test item nominal concentration of 60.5 mg/l and a test item

theoretical oxygen demand (ThOD) of 1.67 mg O2/mg.

The correspondig ThOD applied in the test vessels was 101.2 mg O2/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The biodegradability of Glycerolformal methacrylate exposed to microorganisms derived from activated sludge of a municipal sewage treatment plant was investigated under aerobic static exposure conditions using the Manometric
Respirometry Test OECD 301F.
Biodegradation of Glycerolformal methacrylate based on O2 consumption was calculated to be 40% after 28 days, as compared to the theoretical oxygen demand (ThOD).
The biodegradation of Glycerolformal methacrylate reached 32% at the end of the 10-d window.
Biodegradation of the test item was observed after a lag phase of about 1 day.
The procedure control sodium benzoate reached 77% biodegradation after 14 days, thus confirming the suitability of the inoculum and of the test conditions.
The calculated biodegradation based on DOC measurement reached 37% for Glycerolformal methacrylate and 100% for sodium benzoate. The data essentially
confirm the limited biodegradability of the test item.
Glycerolformal methacrylate did not reach the pass level of 60% for ready biodegradability in the Manometric Respirometry Test, neither within the 10-d
window nor after 28 days of incubation and, therefore, cannot be termed as readily biodegradable.

Executive summary:

The biodegradability of Glycerolformal methacrylate exposed to microorganisms derived from activated sludge of a municipal sewage treatment plant was investigated under aerobic static exposure conditions using the Manometric

Respirometry Test OECD 301F.

Biodegradation of Glycerolformal methacrylate based on O2 consumption was calculated to be 40% after 28 days, as compared to the theoretical oxygen demand (ThOD).

The biodegradation of Glycerolformal methacrylate reached 32% at the end of the 10-d window. Biodegradation of the test item was observed after a lag phase of about 1 day.

The procedure control sodium benzoate reached 77% biodegradation after 14 days, thus confirming the suitability of the inoculum and of the test conditions.

The calculated biodegradation based on DOC measurement reached 37% for Glycerolformal methacrylate and 100% for sodium benzoate. The data essentially confirm the limited biodegradability of the test item.

Glycerolformal methacrylate did not reach the pass level of 60% for ready biodegradability in the Manometric Respirometry Test, neither within the 10-d window nor after 28 days of incubation and, therefore, cannot be termed as readily biodegradable.