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EC number: 213-579-1
CAS number: 987-65-5
The results of the optical density (OD) measured at 570 nm of each
replicate and the calculated % viability of the cells is presented below:
Negative Control (1xPBS):
Standard Deviation (SD)
Positive Control (SDS 5% aq.):
EpiSkinTM SM test of ATP, Di-Na (CAS 987-65-5) has been performed to
predict its irritation potential by measurement of its cytotoxic effect,
as reflected in the MTT assay, according to the OECD Test Guideline No.
439, 28 July 2015.
Disks of EPISKIN (three units) were treated with test item and incubated
for 15 minutes at room temperature. Exposure of test material was
terminated by rinsing with PBS 1x solution. Epidermis units were then
incubated at 37°C for 42 hours in an incubator with 5% CO2. The
viability of each disk was assessed by incubating the tissues for 3
hours with MTT solution at 37°C in 5% CO2 protected from light. The
precipitated formazan was then extracted using acidified isopropanol and
No colour change was observed after three hours of incubation during the
check-test for possible direct MTT reduction with test item. The test
item did not interact with the MTT, therefore additional controls and
data calculations were not necessary.
The test item showed no ability to become coloured in contact with
water. The intrinsic colour of test item is white and therefore
considered to be not able to significantly stain the tissues and lead
false estimate of viability. Furthermore, the test item was completely
removed from the epidermal surface at rinsing period. Additional
controls and data calculations were not necessary.
SDS (5% aq.) and 1×PBS treated (three units / positive and negative
control) epidermis were used as positive and negative controls
respectively. For each treated tissue viability was expressed as a
percentage relative to negative control.
The test chemical is identified as requiring classification and
labelling according to UN GHS (Category 2 or Category 1), if the mean
relative viability after 15 minutes exposure and 42 hours post
incubation is less or equal (=) to 50% of the negative control.
In this in vitro skin irritation test using the EPISKIN model, the test
item ATP, Di-Na did not show significantly reduced cell viability in
comparison to the negative control (mean value: 83 %). All obtained test
item viability results were far above 50 % when compared to the
viability values obtained from the negative control. Therefore the test
item was considered to be non-irritant to skin.
Positive and negative controls showed the expected cell viability values
within acceptable limits. Positive and negative control values were
within the corresponding historical control data ranges.
All assay acceptance criteria were met, the experiment was considered to
The results obtained from this in vitro skin irritation test, using the
EPISKIN model, indicated that the test item reveals no skin irritation
potential under the utilized testing conditions. The test item ATP,
Di-Na (CAS 987-65-5) is considered to be non-irritant to skin and is
therefore not classified (UN GHS No Category / CLP not classified).
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