Registration Dossier

Administrative data

Description of key information

Skin irritation:

The dermal irritation potential of 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) was estimated using OECD QSAR toolbox version 3.3 with logPow as the primary descriptor. The chemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) can be considered to be not irritating to skin and can be classified under the category “Not Classified” as per CLP regulation.

Eye irritation:

The ocular irritation potential of 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) was estimated using OECD QSAR toolbox version 3.3 with logPow as the primary descriptor. The chemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) was estimated to be irritating into the eyes of New Zealand White rabbits. Based on the estimated result 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) can be considered to be irritating to eye and can be classified under the category “Category 2”as per CLP regulation.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records
Reference
Endpoint:
skin irritation: in vivo
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
Justification for type of information:
Data is from OECD QSAR toolbox version 3.3 and QMRF report has been attached
Qualifier:
according to
Guideline:
other: estimated data
Principles of method if other than guideline:
Prediction is done using QSAR Toolbox version 3.3
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (IUPAC name): 3,7-bis(diethylamino)phenoxazin-5-ium acetate
- Molecular formula: C22H29N3O3
- Molecular weight: 383.489 g/mol
- Smiles notation: c1c2nc3ccc(cc3[o+]c2cc(N(CC)CC)c1)N(CC)CC.C(C)(=O)[O-]
-InChl:1S/C20H26N3O.C2H4O2/c1-5-22(6-2)15-9-11-17-19(13-15)24-20-14-16(23(7-3)8-4)10-12-18(20)21-17;1-2(3)4/h9-14H,5-8H2,1-4H3;1H3,(H,3,4)/q+1;/p-1
- Substance type: Organic
- Physical state:
Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
No data available
Type of coverage:
occlusive
Preparation of test site:
shaved
Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
No data available
Duration of treatment / exposure:
4 hours
Observation period:
72 hours
Number of animals:
3
Details on study design:
No data available
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
72 h
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
No skin irritation was observed in treated rabbits.

Estimation method: Takes mode value from the 7 nearest neighbours
Domain  logical expression:Result: In Domain

((((((("a" or "b" )  and "c" )  and ("d" and ( not "e") )  )  and ("f" and ( not "g") )  )  and ("h" and ( not "i") )  )  and ("j" and ( not "k") )  )  and ("l" and "m" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Tertiary aromatic amine by DNA binding by OECD

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Peroxy Acids by Aquatic toxicity classification by ECOSAR

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as No alert found by DNA binding by OASIS v.1.3 ONLY

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Tertiary aromatic amine by DNA binding by OECD

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Michael addition OR Michael addition >> P450 Mediated Activation to Quinones and Quinone-type Chemicals OR Michael addition >> P450 Mediated Activation to Quinones and Quinone-type Chemicals >> Arenes OR No alert found OR SN1 >> Iminium Ion Formation OR SN1 >> Iminium Ion Formation >> Aliphatic tertiary amines OR SN1 >> Nitrenium Ion formation >> Aromatic azo OR SN1 >> Nitrenium Ion formation >> Secondary aromatic amine by DNA binding by OECD

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as No alert found by Protein binding by OASIS v1.3

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as SN1 OR SN1 >> Carbenium ion formation (enzymatic) OR SN1 >> Carbenium ion formation (enzymatic) >> Carbenium ion by Protein binding by OASIS v1.3

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as No alert found by Protein binding by OECD

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as Acylation OR Acylation >> Direct Acylation Involving a Leaving group OR Acylation >> Direct Acylation Involving a Leaving group >> Acetates by Protein binding by OECD

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as Not categorized by Repeated dose (HESS)

Domain logical expression index: "k"

Referential boundary: The target chemical should be classified as Anilines (Hemolytic anemia with methemoglobinemia) Rank A by Repeated dose (HESS)

Domain logical expression index: "l"

Parametric boundary:The target chemical should have a value of log Kow which is >= 2.01

Domain logical expression index: "m"

Parametric boundary:The target chemical should have a value of log Kow which is <= 6.54

Interpretation of results:
other: not irritating
Conclusions:
The chemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) can be considered to be not irritating to skin.
Executive summary:

The dermal irritation potential of 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) was estimated using OECD QSAR toolbox version 3.3 with logPow as the primary descriptor. The chemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) can be considered to be not irritating to skin and can be classified under the category “Not Classified” as per CLP regulation.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records
Reference
Endpoint:
eye irritation: in vivo
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
Justification for type of information:
Data is from OECD QSAR toolbox version 3.3 and QMRF report has been attached
Qualifier:
according to
Guideline:
other: estimated data
Principles of method if other than guideline:
Prediction is done using QSAR Toolbox version 3.3
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (IUPAC name): 3,7-bis(diethylamino)phenoxazin-5-ium acetate
- Molecular formula: C22H29N3O3
- Molecular weight: 383.489 g/mol
- Smiles notation: c1c2nc3ccc(cc3[o+]c2cc(N(CC)CC)c1)N(CC)CC.C(C)(=O)[O-]
-InChl:1S/C20H26N3O.C2H4O2/c1-5-22(6-2)15-9-11-17-19(13-15)24-20-14-16(23(7-3)8-4)10-12-18(20)21-17;1-2(3)4/h9-14H,5-8H2,1-4H3;1H3,(H,3,4)/q+1;/p-1
- Substance type: Organic
Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
No data available
Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
No data available
Duration of treatment / exposure:
single application
Observation period (in vivo):
72 hours
Duration of post- treatment incubation (in vitro):
No data available
Number of animals or in vitro replicates:
3
Details on study design:
No data available
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
72 h
Reversibility:
not specified
Remarks on result:
positive indication of irritation
Irritant / corrosive response data:
Severe eye irritation was observed,

Estimation method: Takes mode value from the 7 nearest neighbours
Domain  logical expression:Result: In Domain

((((((((((((((((((((("a" or "b" )  and ("c" and ( not "d") )  )  and ("e" and ( not "f") )  )  and ("g" and ( not "h") )  )  and "i" )  and "j" )  and ("k" and ( not "l") )  )  and ("m" and ( not "n") )  )  and ("o" and ( not "p") )  )  and ("q" and ( not "r") )  )  and ("s" and ( not "t") )  )  and ("u" and ( not "v") )  )  and ("w" and ( not "x") )  )  and ("y" and ( not "z") )  )  and ("aa" and ( not "ab") )  )  and ("ac" and ( not "ad") )  )  and "ae" )  and "af" )  and "ag" )  and "ah" )  and ("ai" and "aj" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Tertiary aromatic amine by DNA binding by OECD

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Peroxy Acids by Aquatic toxicity classification by ECOSAR

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as Non binder, without OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as Strong binder, OH group by Estrogen Receptor Binding

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Non binder, without OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as Weak binder, OH group by Estrogen Receptor Binding

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as Non binder, without OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as Non binder, MW>500 by Estrogen Receptor Binding

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as No alert found by DNA binding by OASIS v.1.3 ONLY

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as No alert found by DNA binding by OASIS v.1.3 ONLY

Domain logical expression index: "k"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Tertiary aromatic amine by DNA binding by OECD

Domain logical expression index: "l"

Referential boundary: The target chemical should be classified as SN1 >> Nitrenium Ion formation >> Aromatic azo by DNA binding by OECD

Domain logical expression index: "m"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Tertiary aromatic amine by DNA binding by OECD

Domain logical expression index: "n"

Referential boundary: The target chemical should be classified as SN1 >> Iminium Ion Formation by DNA binding by OECD

Domain logical expression index: "o"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Tertiary aromatic amine by DNA binding by OECD

Domain logical expression index: "p"

Referential boundary: The target chemical should be classified as Michael addition >> P450 Mediated Activation to Quinones and Quinone-type Chemicals >> Arenes by DNA binding by OECD

Domain logical expression index: "q"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg AND (!Undefined)Group CN Lipid Solubility < 0.4 g/kg AND Group All Melting Point > 200 C AND Group CN Aqueous Solubility < 0.1 g/L AND Group CN Molecular Weight > 290 g/mol by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "r"

Referential boundary: The target chemical should be classified as (!Undefined)Group CNHal Lipid Solubility < 400 g/kg by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "s"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg AND (!Undefined)Group CN Lipid Solubility < 0.4 g/kg AND Group All Melting Point > 200 C AND Group CN Aqueous Solubility < 0.1 g/L AND Group CN Molecular Weight > 290 g/mol by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "t"

Referential boundary: The target chemical should be classified as Group All Aqueous Solubility < 0.000005 g/L by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "u"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg AND (!Undefined)Group CN Lipid Solubility < 0.4 g/kg AND Group All Melting Point > 200 C AND Group CN Aqueous Solubility < 0.1 g/L AND Group CN Molecular Weight > 290 g/mol by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "v"

Referential boundary: The target chemical should be classified as Group C Aqueous Solubility < 0.0001 g/L by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "w"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg AND (!Undefined)Group CN Lipid Solubility < 0.4 g/kg AND Group All Melting Point > 200 C AND Group CN Aqueous Solubility < 0.1 g/L AND Group CN Molecular Weight > 290 g/mol by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "x"

Referential boundary: The target chemical should be classified as Group C Aqueous Solubility < 0.0005 g/L by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "y"

Referential boundary: The target chemical should be classified as Aromatic amines by Skin irritation/corrosion Inclusion rules by BfR

Domain logical expression index: "z"

Referential boundary: The target chemical should be classified as (Hydro)Peroxides by Skin irritation/corrosion Inclusion rules by BfR

Domain logical expression index: "aa"

Referential boundary: The target chemical should be classified as Non-Metals by Groups of elements

Domain logical expression index: "ab"

Referential boundary: The target chemical should be classified as Alkali Earth OR Metalloids by Groups of elements

Domain logical expression index: "ac"

Referential boundary: The target chemical should be classified as Group 14 - Carbon C AND Group 15 - Nitrogen N AND Group 16 - Oxygen O by Chemical elements

Domain logical expression index: "ad"

Referential boundary: The target chemical should be classified as Group 16 - Sulfur S by Chemical elements

Domain logical expression index: "ae"

Referential boundary: The target chemical should be classified as Acetoxy AND Aromatic amine AND Aryl AND Carboxylic acid AND Fused carbocyclic aromatic AND Fused heterocyclic aromatic by Organic Functional groups ONLY

Domain logical expression index: "af"

Referential boundary: The target chemical should be classified as Acetoxy AND Aromatic amine AND Fused carbocyclic aromatic AND Fused heterocyclic aromatic AND Overlapping groups by Organic Functional groups (nested) ONLY

Domain logical expression index: "ag"

Referential boundary: The target chemical should be classified as Acetoxy AND Aromatic amine AND Fused carbocyclic aromatic AND Fused heterocyclic aromatic AND Overlapping groups by Organic Functional groups (nested) ONLY

Domain logical expression index: "ah"

Referential boundary: The target chemical should be classified as Acetoxy AND Aromatic amine AND Fused carbocyclic aromatic AND Fused heterocyclic aromatic AND Overlapping groups by Organic Functional groups (nested) ONLY

Domain logical expression index: "ai"

Parametric boundary:The target chemical should have a value of log Kow which is >= 0.933

Domain logical expression index: "aj"

Parametric boundary:The target chemical should have a value of log Kow which is <= 7.57

Interpretation of results:
Category 2 (irritating to eyes) based on GHS criteria
Conclusions:
The chemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) was estimated to be irritating into the eyes of New Zealand White rabbits. Based on the estimated result 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916 -07 -7) can be considered to be irritating to eye.
Executive summary:

The ocular irritation potential of 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) was estimated using OECD QSAR toolbox version 3.3 with logPow as the primary descriptor. The chemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) was estimated to be irritating into the eyes of New Zealand White rabbits. Based on the estimated result 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916 -07 -7) can be considered to be irritating to eye and can be classified under the category “Category 2” as per CLP regulation.

Endpoint conclusion
Endpoint conclusion:
adverse effect observed (irritating)

Respiratory irritation

Endpoint conclusion
Endpoint conclusion:
no study available

Additional information

Skin irritation:

Various studieshas been investigated for the test chemical3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) to observe the potential for dermal irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits and guinea pigs for target chemical3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) and its structurally similar read across substances7-Diethylamino-4-methylcoumarin (CAS No: 91-44-1)and Rhodamine B (CAS No: 81-88-9).The predicted data using the OECD QSAR toolbox has also been compared with the experimental data and summarized as below;

 

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the skin irritation potential was estimated for test chemical3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7)  .The chemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) is estimated to be not irritating to skin of New Zealand White rabbits.

 

The B.M. Hausen and M. Berger {Contact Dermatitis1989: 21: 141-147} conducted skin irritation study of read across substance7-Diethylamino-4-methylcoumarin (CAS No: 91-44-1)in guinea pigs.The10 female Pirbright-White guinea pigs were treated with an emulsion prepared from FCA and physiological saline but without the test chemical and applied on a semicircular arc on the clipped or shaved shoulder area (4*6 cm) from left to right in such a way that whole emulsion was used up for the animals.1 day before the challenge exposure, teh guinea pigs were treated with 7-Diethylamino-4-methylcoumarin (CAS No: 91-44-1)  by applying different concentrations of the test chemical (10%, 3% and 1%) to the right flank.The results of the challenge exposure were observed after 24 hours. A (+) reaction was considered as threshold of irritation. The mean irritation index of 7-Diethylamino-4-methylcoumarin was 0.0 after 24 hours. Hence, the test material 7-Diethylamino-4-methylcoumarin (CAS No: 91-44-1) was considered to be not irritating to guinea pigs.

 

Above results were further supported by dermal irritation carried out byJAMES E. FULTON, JR { J. Soc. Cosmet. Chem., 40, 321-333 (November/December 1989)} for read across substanceRhodamine B(CAS No: 81-88-9).The test uses a single rabbit ear to indicate the Comedogenicity and irritancy of Rhodamine B. Rhodamine B was mixed in propylene glycol at a 9 to 1 dilution for testing unless otherwise indicated (10% concentration). A colony of New Zealand albino rabbits that have genetically good ears and free from mites were used. Three rabbits, weighing two to three kilograms, were used for each assay. Animals were housed singly in suspended cages and fed Purina Rabbit Chow and water ad libitum. Animals were maintained on a 12-hour light and 12-hour dark cycle. A dose of 1 ml of the test material was applied and spread once daily to the entire inner surface of once for five days per week for two weeks. The opposite untreated ear of each animal served as an untreated control. The irritancy produced by repeated application of the chemical on the surface epidermis in the rabbit ear is evaluated on a scale of 0 to 5. The grades are summarized as follows:  0 = No irritation; 1 = few scales, no Erythema; 2 = diffuse scaling, no Erythema; 3 = Generalized scaling with Erythema; 4 = Scaling, Erythema and Edema; 5 = Epidermal necrosis and slough. Rhodamine B falls under Grade 0 (no irritation observed). Hence it can be concluded that Rhodamine B was not irritating to rabbit ears.

 

Thus on the basis of available data for thetarget chemical3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) and its structurally similar read across substances7-Diethylamino-4-methylcoumarin (CAS No: 91-44-1)and Rhodamine B (CAS No: 81-88-9),it can be concluded thatchemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) is unable to cause skin irritation and considered as not irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.

 

 

Eye irritation:

In different studies,the test chemical3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) has been investigated for potential for ocular irritationto a greater or lesser extent. The studies are based on in vivo experiments in rabbits for target chemical3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) its structurally similar read across substances Rhodamine B(CAS No: 81-88-9)andN,N-dimethylaniline (CAS no: 121-69-7).The predicted data using the OECD QSAR toolbox has also been compared with the experimental data and summarized as below;

 

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the eye irritation potential was estimated for test chemical3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) .The chemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) is estimated to be severely irritating to eye of New Zealand White rabbits.

 

The Daniel J. Dire & Joseph A. Wilkinson{Journal of Toxicology: Clinical Toxicology, 25:7, 603-607; 1987} conducted an eye irritation study for read across substanceRhodamine B(CAS No: 81-88-9).The study reported the evaluation of 17 patients who were exposed to aerosolized Rhodamine B. Approximately 10 lbs =(4.53kg) 4530000mg of Rhodamine B powder was spilled onto the floor of a vehicle maintenance shop. A cleanup crew began to sweep up the powder from the floor. This resulted in an aerosol of Rhodamine B dust that dispersed throughout the building contaminating the hair, skin, eyes, and clothing of most of the shop employees. Sixteen of the patients (94%) complained of symptoms such as redness, burning sensation..etc. One patient (6%) denied any symptoms despite being exposed to the dust for 15 minutes without a surgical mask. The most frequently reported symptom was burning of the eyes which occurred in 82% of the patients. Four of the patients (24%) were placed on humidified oxygen because of respiratory symptoms. Five of the patients (29%) underwent irrigation of their eyes secondary to eye burning. After being discharged from the hospital, follow up was maintained. Ten of the patients (59%) reported that their symptoms had resolved within 4 hours of their exposure to the dust. One patient (6%) reported that his symptoms lasted 12 hours and 2 patients (12%) reported their symptoms lasted 24 hours. Three of the patients (18%) could not remember the duration of their symptoms. Burning of eyes was observed in 82% of the patients. Based on these effects Rhodamine B may be considered as irritating to eyes.

 

The above results were further supported by the an acute eye irritation study performed by Henry F. Smyth et,al., {American Industrial Hygiene Association Journal, 30:5, 470-476, 1969}of read across chemical N,N-dimethylaniline (CAS no: 121-69-7)in rabbits. Eye injury in rabbits is recorded in a 10- grade ordinal series and is based upon the degree of corneal necrosis that results from instillation of various volumes and concentrations of chemical. Grade 1 indicates at most a very small area of necrosis resulting from 0.5 ml of undiluted chemical in the eye. Grade 5 indicates a so-called severe burn from 0.005 ml, and Grade 10 indicates a severe burn from 0.5 ml of a 1% solution in water or propylene glycol. The Primary Irritation score after 24 hours for N,N-dimethylaniline (CAS no: 121-69-7)was Grade 5. Thus the chemical N,N-dimethylaniline (CAS no: 121-69-7) was considered to be irritating to the rabbit skin.

 

Thus on the basis of available data for thetarget chemical3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) and its structurally similar read across substancesRhodamine B(CAS No: 81-88-9)andN,N-dimethylaniline (CAS no: 121-69-7),it can be concluded thatchemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) is able to cause eye irritation and considered as irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Category 2”.

Justification for classification or non-classification

The skin and eye irritation potential of test chemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) and its structurally similar read across substanceswere observed in various studies. The results obtained from these studies indicate that the chemical 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) is unlikely to cause skin irritation but can cause severe irreversible eye damage . Hence 3,7-bis(diethylamino)phenoxazin-5-ium acetate (CAS No: 79916-07-7) can be classified under the category “Not Classified” for skin and “Category 2” for eye as per CLP.